Abstract
N-acetyl-α-hydroxy-β-oxotryptamine (1) along with N-acetyl-β-oxotryptamine (2) and pimprinine (3) were isolated from the culture broth of Streptomyces sp. 80H647. Compound 1 was found to be a racemate via X-ray diffraction analysis and the enantiomers were successfully purified by chiral-phase HPLC. The absolute configuration was assigned by comparison of the calculated and experimental ECD spectra. The α-hydroxy moiety of 1 was vital for cytotoxicity against different cancer cell lines.
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Acknowledgements
We thank Dr. T. Kawamura for the ROS assay. The computer calculation using Gaussian 16 was performed using the HOKUSAI supercomputer at RIKEN.
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Lopez, J.A.V., Nogawa, T., Futamura, Y. et al. N-Acetyl-α-hydroxy-β-oxotryptamine, a racemic natural product isolated from Streptomyces sp. 80H647. J Antibiot 74, 477–479 (2021). https://doi.org/10.1038/s41429-021-00420-7
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DOI: https://doi.org/10.1038/s41429-021-00420-7
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