Fig. 6

Function of Sema4C in fear and fear memory on dendritic structural remodeling ex vivo. a ELISA-based analysis of RhoA activation in cultured mouse hippocampal neurons upon exposure to Sema4C (150 nM for 15 min); n = 4 independent culture experiments); Student’s t-test, *P < 0.05 as compared to vehicle-treated cultures. b–d Typical examples (b) and quantitative analysis of total dendritic length (c) and number of dendritic crossing (d; range 50–100µm from the soma) of EGFP-labeled hippocampal neurons from wild-type mice at 10 days in vitro (DIV). e, f Typical examples (e) and quantitative summary (f) of morphometric analysis of EGFP-labeled synaptic spines on wild-type hippocampal neurons at 10 DIV upon treatment with vehicle, Sema4C (150 nM), Y-27632 (3.3 µM) or Sema4C (150 nM) + Y-27632 (3.3 µM) for 24 h. In panels c, d, f: one-way ANOVA with post hoc Tukey’s test; n = 11–12 neurons per condition, 3 independent culture preparations. *P < 0.05 as compared to vehicle-treated cultures, ^#P < 0.05 as compared to Sema4C-treated neurons. Error bars represent SEM. Scale bars represent 100 µm (a, c), 20 µm (g) and 5 µm (j), respectively