Fig. 3

Analysis of function of Plexin-B2 in brain regions involved in fear and fear memory. a Schematic representation of strategy for generation of mice lacking plxnb2 conditionally in forebrain excitatory neurons in a Tamoxifen-inducible manner. b Immunohistochemistry demonstrating loss of Plexin-B2 expression in CA1 neurons on hippocampus 2 weeks post-Tamoxifen administration to adult CaMK-PB2^−/− mice. c–e Analysis of contextual fear memory over recent (2 days) and remote (36 days) stages post-fear conditioning in CaMK-PB2^−/− mice and their control littermates (PB2^fl/fl, c; n = 8 mice per group), or in mice constitutively lacking Plxnb1 (Plexin-B1^−/− mice) and their control wild-type littermates (d; n = 8 mice per group), or in mice globally lacking Sema4c (Sema4C^−/− mice) and their control wild-type littermates (e; n = 7 mice per group) Student’s t-test. P < 0.05 indicated by ^† as compared to the corresponding control (wild-type) littermates. Error bars represent SEM. Scale bars represent 500 µm (b)