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Frozen in action: cryo-EM structure of a GPCR–G-protein complex

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Interaction with heterotrimeric G proteins is a hallmark of G-protein-coupled receptor (GPCR) family members, and it is the key step for a diverse range of cell-signaling cascades. A recent cryo-EM structure of the human calcitonin receptor (CTR) in complex with a G-protein heterotrimer reveals novel insights into receptor–G-protein coupling.

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Figure 1: Progress in the structural analysis of GPCR signaling complexes by EM.
Figure 2: Structural comparison of the β2AR–G-protein complex and the CTR–G-protein complex.
Figure 3: Superimposition of the structures of the β2AR–G-protein complex and the CTR–G-protein complex.

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Acknowledgements

The research program in A.K.S.'s laboratory is supported by the Wellcome Trust DBT India Alliance (IA/I/14/1/501285), the Department of Biotechnology (DBT), the Department of Science and Technology (DST), the Council for Scientific and Industrial Research (CSIR) and the Institute of Technology, Kanpur.

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Authors and Affiliations

  1. Hemlata Dwivedi and Arun K. Shukla are in the Department of Biological Sciences and Bioengineering, Mithu Baidya, Indian Institute of Technology, Kanpur, India.,

    Mithu Baidya, Hemlata Dwivedi & Arun K Shukla

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  1. Mithu Baidya
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  2. Hemlata Dwivedi
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  3. Arun K Shukla
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Correspondence to Arun K Shukla.

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Baidya, M., Dwivedi, H. & Shukla, A. Frozen in action: cryo-EM structure of a GPCR–G-protein complex. Nat Struct Mol Biol 24, 500–502 (2017). https://doi.org/10.1038/nsmb.3418

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