Skip to main content
SpringerLink
Log in

Separase–securin complex: a cunning way to control chromosome segregation

  • News & Views
  • Published:

From Nature Structural & Molecular Biology

View current issue Submit your manuscript

Separases are crucial cell cycle proteases that control the metaphase-to-anaphase transition by cleaving chromosomal cohesin rings. Two new high-resolution structures of separase bound by its inhibitory chaperone securin illustrate intriguing regulatory mechanisms.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: Ribbon representations of the budding yeast S. cerevisiae separase–securin complex (top) and the nematode worm C. elegans complex (bottom).
Figure 2: Active site diagrams of separase in active and inactive configurations.

References

  1. Uhlmann, F., Lottspeich, F. & Nasmyth, K. Nature 400, 37–42 (1999).

    Article  CAS  Google Scholar 

  2. Uhlmann, F., Wernic, D., Poupart, M.-A., Koonin, E.V. & Nasmyth, K. Cell 103, 375–386 (2000).

    Article  CAS  Google Scholar 

  3. Waizenegger, I.C., Hauf, S., Meinke, A. & Peters, J.-M. Cell 103, 399–410 (2000).

    Article  CAS  Google Scholar 

  4. Luo, S. & Tong, L. Nature 542, 255–259 (2017).

    Article  CAS  Google Scholar 

  5. Boland, A. et al. Nat. Struct. Mol. Biol. 24, 414–418 (2017).

    Article  CAS  Google Scholar 

  6. McGrew, J.T., Goetsch, L., Byers, B. & Baum, P. Mol. Biol. Cell 3, 1443–1454 (1992).

    Article  CAS  Google Scholar 

  7. Uzawa, S., Samejima, I., Hirano, T., Tanaka, K. & Yanagida, M. Cell 62, 913–925 (1990).

    Article  CAS  Google Scholar 

  8. Cohen-Fix, O., Peters, J.-M., Kirschner, M.W. & Koshland, D. Genes Dev. 10, 3081–3093 (1996).

    Article  CAS  Google Scholar 

  9. Funabiki, H. et al. Nature 381, 438–441 (1996).

    Article  CAS  Google Scholar 

  10. Zou, H., McGarry, T.J., Bernal, T. & Kirschner, M.W. Science 285, 418–422 (1999).

    Article  CAS  Google Scholar 

  11. Jensen, S., Segal, M., Clarke, D.J. & Reed, S.I. J. Cell Biol. 152, 27–40 (2001).

    Article  CAS  Google Scholar 

  12. Hornig, N.C.D., Knowles, P.P., McDonald, N.Q. & Uhlmann, F. Curr. Biol. 12, 973–982 (2002).

    Article  CAS  Google Scholar 

  13. Waizenegger, I., Giménez-Abián, J.F., Wernic, D. & Peters, J.-M. Curr. Biol. 12, 1368–1378 (2002).

    Article  CAS  Google Scholar 

  14. Funabiki, H., Kumada, K. & Yanagida, M. EMBO J. 15, 6617–6628 (1996).

    Article  CAS  Google Scholar 

  15. Stratmann, R. & Lehner, C.F. Cell 84, 25–35 (1996).

    Article  CAS  Google Scholar 

  16. Nagao, K. & Yanagida, M. Genes Cells 11, 247–260 (2006).

    Article  CAS  Google Scholar 

  17. Viadiu, H., Stemmann, O., Kirschner, M.W. & Walz, T. Nat. Struct. Mol. Biol. 12, 552–553 (2005).

    Article  CAS  Google Scholar 

  18. Lin, Z., Luo, X. & Yu, H. Nature 532, 131–134 (2016).

    Article  CAS  Google Scholar 

  19. Bachmann, G. et al. Open Biol. 6, 160032 (2016).

    Article  Google Scholar 

  20. Sullivan, M., Hornig, N.C.D., Porstmann, T. & Uhlmann, F. J. Biol. Chem. 279, 1191–1196 (2004).

    Article  CAS  Google Scholar 

  21. Stemmann, O., Zou, H., Gerber, S.A., Gygi, S.P. & Kirschner, M.W. Cell 107, 715–726 (2001).

    Article  CAS  Google Scholar 

  22. Hornig, N.C.D. & Uhlmann, F. EMBO J. 23, 3144–3153 (2004).

    Article  CAS  Google Scholar 

  23. Sun, Y. et al. Cell 137, 123–132 (2009).

    Article  CAS  Google Scholar 

  24. Hellmuth, S. et al. Mol. Cell 58, 495–506 (2015).

    Article  CAS  Google Scholar 

  25. Sullivan, M. & Uhlmann, F. Nat. Cell Biol. 5, 249–254 (2003).

    Article  CAS  Google Scholar 

  26. Kudo, N.R. et al. Cell 126, 135–146 (2006).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Martin R. Singleton is at the Structural Biology of Chromosome Segregation Laboratory, The Francis Crick Institute, London, UK.,

    Martin R Singleton

  2. Frank Uhlmann is at the Chromosome Segregation Laboratory, The Francis Crick Institute, London, UK.,

    Frank Uhlmann

Authors
  1. Martin R Singleton
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Frank Uhlmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Martin R Singleton or Frank Uhlmann.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Singleton, M., Uhlmann, F. Separase–securin complex: a cunning way to control chromosome segregation. Nat Struct Mol Biol 24, 337–339 (2017). https://doi.org/10.1038/nsmb.3393

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nsmb.3393

  • Springer Nature America, Inc.

This article is cited by

Search

Navigation