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Single-virus tracking uncovers the missing link between HIV integration site location and viral gene expression

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The site of HIV genome integration is likely a contributing factor in viral gene expression, but such context-specific effects are difficult to demonstrate at the population level. A new approach overcomes this obstacle by tracking individual, barcoded viruses to investigate the relationship between integration site location and the corresponding viral transcription, thereby providing insights essential for understanding HIV production, latency and reactivation.

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Figure 1: Single-virus tracking by B-HIVE.
Figure 2: HIV integration site location affects viral gene expression and susceptibility to LRAs.

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Authors and Affiliations

  1. Angela Ciuffi, Sara Cristinelli and Sylvie Rato are at the Institute of Microbiology, Lausanne University Hospital (CHUV) and University of Lausanne, Lausanne, Switzerland.,

    Angela Ciuffi, Sara Cristinelli & Sylvie Rato

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  1. Angela Ciuffi
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  2. Sara Cristinelli
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  3. Sylvie Rato
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Corresponding authors

Correspondence to Angela Ciuffi, Sara Cristinelli or Sylvie Rato.

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The authors declare no competing financial interests.

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Ciuffi, A., Cristinelli, S. & Rato, S. Single-virus tracking uncovers the missing link between HIV integration site location and viral gene expression. Nat Struct Mol Biol 24, 8–11 (2017). https://doi.org/10.1038/nsmb.3358

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