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Discovery and annotation of long noncoding RNAs

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Abstract

Recent advances in RNA-sequencing technologies have led to the discovery of thousands of previously unannotated noncoding transcripts, including many long noncoding RNAs (lncRNAs) whose functions remain largely unknown. Here we discuss considerations and best practices in lncRNA identification and annotation, which we hope will foster functional and mechanistic exploration.

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Authors and Affiliations

  1. John S. Mattick is at the Garvan Institute of Medical Research, Sydney, New South Wales, Australia, and St Vincent's Clinical School, University of New South Wales, Sydney, New South Wales, Australia.,

    John S Mattick

  2. John L. Rinn is at the Department of Stem Cell and Regenerative Biology, the Department of Pathology, Harvard University, Cambridge, Massachusetts, USA, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA, and the Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.,

    John L Rinn

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  1. John S Mattick
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  2. John L Rinn
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Correspondence to John S Mattick or John L Rinn.

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The authors declare no competing financial interests.

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Mattick, J., Rinn, J. Discovery and annotation of long noncoding RNAs. Nat Struct Mol Biol 22, 5–7 (2015). https://doi.org/10.1038/nsmb.2942

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