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Genetic code expansion

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Expanding the genetic code by ribosome-mediated introduction of non-natural amino acids into proteins requires engineering of aminoacyl-tRNA synthetase–tRNA pairs. Structural information for pairs specific for tyrosine reveals how this goal may be achieved on a rational basis.

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Figure 1: Different recognition patterns of tRNATyr identity determinants by TyrRSs from archaea and eukarya (top) and from eubacteria (bottom).

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Authors and Affiliations

  1. Département Mécanismes et Macromolécules de la Synthèse Protéique et Cristallogenèse, UPR 9002 du CNRS, Institut de Biologie Moléculaire et Cellulaire, 15 rue René Descartes, Strasbourg, 67084, Cedex, France

    Richard Giegé

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  1. Richard Giegé
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Giegé, R. Genetic code expansion. Nat Struct Mol Biol 10, 414–416 (2003). https://doi.org/10.1038/nsb0603-414

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