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The structural basis for spectral variations in green fluorescent protein

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Based on structures of five mutants of green fluorescent protein (GFP), we describe two conformational variants with differing charge distribution that explain the published absorption and fluorescence spectra of GFP mutants.

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Authors and Affiliations

  1. Protein Structure Section, Macromolecular Structure Laboratory, NCI-Frederick Cancer and Research Development Center, ABL-Basic Research Program, Frederick, Maryland, 21702-1201, USA

    Gottfried J. Palm, Alexander Zdanov & Alexander Wlodawer

  2. Human Retrovirus Section, Molecular Virology and Carcinogenesis Laboratory, NCI-Frederick Cancer and Research Development Center, ABL-Basic Research Program, Frederick, Maryland, 21702-1201, USA

    George A. Gaitanaris, Roland Stauber & George N. Pavlakis

Authors
  1. Gottfried J. Palm
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  2. Alexander Zdanov
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  3. George A. Gaitanaris
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  4. Roland Stauber
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  5. George N. Pavlakis
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  6. Alexander Wlodawer
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Palm, G., Zdanov, A., Gaitanaris, G. et al. The structural basis for spectral variations in green fluorescent protein. Nat Struct Mol Biol 4, 361–365 (1997). https://doi.org/10.1038/nsb0597-361

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  • DOI: https://doi.org/10.1038/nsb0597-361

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