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Epitaxial growth of protein crystals on lipid layers

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Abstract

The growth of three–dimensional protein crystals is seeded by two–dimensional crystals formed on lipid layers. Such crystallization occurs faster and at lower precipitant and protein concentrations than conventional crystal growth. This approach may also allow the crystallization of proteins that resist attempts at crystal growth by other means.

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Authors and Affiliations

  1. Department of Cell Biology, Stanford University School of Medicine, Stanford, California, 94305, USA

    Aled M. Edward, Seth A. Darst, Yang Li & Roger D. Kornberg

  2. Department of Pathology, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada, L8N 3Z5

    Aled M. Edward & Sally A. Hemming

  3. Rockefeller University, Box 224, 1230 York Avenue, New York, New York, 10021, USA

    Seth A. Darst

Authors
  1. Aled M. Edward
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  2. Seth A. Darst
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  3. Sally A. Hemming
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  4. Yang Li
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  5. Roger D. Kornberg
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Edward, A., Darst, S., Hemming, S. et al. Epitaxial growth of protein crystals on lipid layers. Nat Struct Mol Biol 1, 195–197 (1994). https://doi.org/10.1038/nsb0394-195

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  • DOI: https://doi.org/10.1038/nsb0394-195

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