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Experience-dependent expression of miR-132 regulates ocular dominance plasticity

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Abstract

miR-132 is a CREB-induced microRNA that is involved in dendritic spine plasticity. We found that visual experience regulated histone post-translational modifications at a CRE locus that is important for miR-212 and miR-132 cluster transcription, and regulated miR-132 expression in the visual cortex of juvenile mice. Monocular deprivation reduced miR-132 expression in the cortex contralateral to the deprived eye. Counteracting this miR-132 reduction with an infusion of chemically modified miR-132 mimic oligonucleotides completely blocked ocular dominance plasticity.

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Figure 1: Visual stimulation induces histone mark modifications on specific CRE loci close to the miR-132 coding sequence and activates mature and primary miR-132 expression.
Figure 2: miR-132 downregulation after monocular deprivation regulates ODP in juvenile mice.

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Acknowledgements

We thank L. Dolfi and F. Cremisi for help with ISH. This work was supported by European Union 7th Framework Program (FP2007–2013) grant agreements 223326 and 223524, EXTRAPLAST IIT project, Telethon project GGP09196 and MIUR EpiGen.

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Authors and Affiliations

  1. Istituto di Neuroscienze Consiglio Nazionale delle Ricerche, Pisa, Italy

    Paola Tognini, Elena Putignano, Alessandro Coatti & Tommaso Pizzorusso

  2. Scuola Normale Superiore, Pisa, Italy

    Paola Tognini, Elena Putignano & Alessandro Coatti

  3. Dipartimento di Psicologia, Università di Firenze, Firenze, Italy

    Tommaso Pizzorusso

Authors
  1. Paola Tognini
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  2. Elena Putignano
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  3. Alessandro Coatti
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  4. Tommaso Pizzorusso
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Contributions

P.T. performed all of the experiments and wrote the manuscript. E.P. performed electrophysiology, ChIP and western blots. A.C. performed ChIP. T.P. wrote the manuscript and supervised the project.

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Correspondence to Tommaso Pizzorusso.

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The authors declare no competing financial interests.

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Supplementary Figures 1–10 and Methods (PDF 509 kb)

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Tognini, P., Putignano, E., Coatti, A. et al. Experience-dependent expression of miR-132 regulates ocular dominance plasticity. Nat Neurosci 14, 1237–1239 (2011). https://doi.org/10.1038/nn.2920

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