Understanding neuronal integration comes a step closer to reality with the development of a crystal-based, beam-steering microscope for uncaging neurotransmitters, which will permit experimental interrogation of the spatiotemporal interactions between the thousands of synapses a neuron receives.
References
Hausser, M., Spruston, N. & Stuart, G.J. Science 290, 739–744 (2000).
Magee, J.C. Nat. Rev. Neurosci. 1, 181–190 (2000).
Shoham, S., O'Connor, D.H., Sarkisov, D.V. & Wang, S.S.-H. Nat. Methods 2, 837–843 (2005).
Nerbonne, J.M. Curr. Opin. Neurobiol. 6, 379–386 (1996).
Polsky, A., Mel, B.W. & Schiller, J. Nat Neurosci. 7, 621–627 (2004).
Kaplan, J.H., Forbush, B. & Hoffman, J.F. Biochemistry 17, 1929–1935 (1978).
Dantzig, J.A., Higuchi, H. & Goldman, Y.E. Methods Enzymol. 291, 307–348 (1998).
Katz, B. & Miledi, R. Proc. R. Soc. Lond. B 161, 496–503 (1965).
Adams, S.R., Kao, J.P.Y., Grynkiewicz, G., Minta, A. & Tsien, R.Y. J. Am. Chem. Soc. 110, 3212–3220 (1988).
Kaplan, J.H. & Ellis-Davies, G.C.R. Proc. Natl. Acad. Sci. USA 85, 6571–6575 (1988).
Milburn, T. et al. Biochemistry 28, 49–55 (1989).
Wieboldt, R. et al. Proc. Natl. Acad. Sci. USA 91, 8752–8756 (1994).
Ellis-Davies, G.C.R. in Imaging in Neuroscience and Development: A Laboratory Manual 2nd edn. (eds. Yuste, R. & Konnerth, A.) 367–373 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2005).
Matsuzaki, M. et al. Nat. Neurosci. 4, 1086–1092 (2001).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Haydon, P., Ellis-Davies, G. Ultrahigh-speed photochemical stimulation of neurons. Nat Methods 2, 811–812 (2005). https://doi.org/10.1038/nmeth1105-811
Issue Date:
DOI: https://doi.org/10.1038/nmeth1105-811
- Springer Nature America, Inc.
We’re sorry, something doesn't seem to be working properly.
Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.