Skip to main content
SpringerLink
Log in

Single-molecule mountains yield nanoscale cell images

  • News & Views
  • Published:

From Nature Methods

View current issue Submit your manuscript

Methods to simultaneously localize the positions of multiple single fluorophores by precisely determining their individual positions are now yielding impressive gains in fluorescence microscopy resolution.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: Measuring mountain peaks for superresolution imaging.

References

  1. Ambrose, W.P., Basché, T. & Moerner, W.E. J. Chem. Phys. 95, 7150–7163 (1991).

    Article  CAS  Google Scholar 

  2. Kim, S.Y., Gitai, Z., Kinkhabwala, A., Shapiro L. & Moerner, W.E. Proc. Natl. Acad. Sci. USA 103, 10929–10934 (2006).

    Article  CAS  Google Scholar 

  3. Gelles, J., Schnapp B.J. & Sheetz, M.P. Nature 331, 450–453 (1988).

    Article  CAS  Google Scholar 

  4. Güttler, F., Irngartinger, T., Plakhotnik, T., Renn, A. & Wild, U.P. Chem. Phys. Lett. 217, 393–397 (1994).

    Article  Google Scholar 

  5. van Oijen, A.M., Kohler, J., Schmidt, J., Muller, M. & Brakenhoff, G.J. Chem. Phys. Lett. 292, 183–187 (1998).

    Article  CAS  Google Scholar 

  6. Thompson, R.E., Larson, D.R. & Webb, W.W. Biophys. J. 82, 2775–2783 (2002).

    Article  CAS  Google Scholar 

  7. Yildiz, A. et al. Science 300, 2061–2065 (2003).

    Article  CAS  Google Scholar 

  8. Hell, S.W., Jakobs S. & Kastrup, L. Appl. Phys. A 77, 859–860 (2003).

    Article  CAS  Google Scholar 

  9. Qu, X., Wu, D., Mets, L. & Scherer, N.F. Proc. Natl. Acad. Sci. USA 101, 11298–11303 (2003).

    Article  Google Scholar 

  10. Betzig, E. et al. Science Express; published online 10 August 2006.

    Google Scholar 

  11. Lidke, K.A., Rieger, B., Jovin, T.M. & Heintzmann, R. Opt. Express 13, 7052–7062 (2005).

    Article  Google Scholar 

  12. Na, X., Sims, P.A., Chen, P. & Xie, X.S. J. Phys. Chem. B 109, 24220–24224 (2005).

    Article  Google Scholar 

  13. Rust, M.J., Bates, M. & Zhuang, X. Nat. Methods 3, 793–795 (2006).

    Article  CAS  Google Scholar 

  14. Willig, K.I. et al. Nat. Methods 3, 721–723 (2006).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. the Department of Chemistry, Stanford University, Stanford, 94305, California, USA

    W E Moerner

Authors
  1. W E Moerner
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Moerner, W. Single-molecule mountains yield nanoscale cell images. Nat Methods 3, 781–782 (2006). https://doi.org/10.1038/nmeth1006-781

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth1006-781

  • Springer Nature America, Inc.

This article is cited by

Search

Navigation