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QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ

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Figure 1: Three-dimensional dSTORM imaging of β-III-tubulin–labeled microtubules in neuronal cells.

References

  1. Rust, M.J., Bates, M. & Zhuang, X. Nat. Methods 3, 793–795 (2006).

    Article  CAS  Google Scholar 

  2. Hess, S.T., Girirajan, T.P. & Mason, M.D. Biophys. J. 91, 4258–4272 (2006).

    Article  CAS  Google Scholar 

  3. Betzig, E. et al. Science 313, 1642–1645 (2006).

    Article  CAS  Google Scholar 

  4. Huang, B., Jones, S.A., Brandenburg, B. & Zhuang, X. Nat. Methods 5, 1047–1052 (2008).

    Article  CAS  Google Scholar 

  5. Huang, B., Wang, W., Bates, M. & Zhuang, X. Science 319, 810–813 (2008).

    Article  CAS  Google Scholar 

  6. Wolter, S. et al. J. Microsc. 237, 12–22 (2010).

    Article  CAS  Google Scholar 

  7. Hedde, P.N., Fuchs, J., Oswald, F., Wiedenmann, J. & Nienhaus, G. U. Nat. Methods 6, 689–690 (2009).

    Article  CAS  Google Scholar 

  8. Högbom, J.A. Astron. Astrophys. 15 (Suppl.), 417–426 (1974).

    Google Scholar 

  9. Stuurman, N., Amodaj, N. & Vale, R.D. Microscopy Today 15, 42–43 (2007).

    Article  Google Scholar 

  10. Heilemann, M. et al. Angew. Chem. Int. Edn Engl. 47, 617–6176 (2008).

    Article  Google Scholar 

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Acknowledgements

We thank J. Enninga for assistance with experiments, and T. Duong and R. Fesce for helpful insight in the preparation of the manuscript.

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Authors and Affiliations

  1. Unidade de Biofísica e Expressão Genética, Instituto de Medicina Molecular, Faculdade de Medicina Universidade de Lisboa, Lisboa, Portugal

    Ricardo Henriques & Musa M Mhlanga

  2. Institut Pasteur, Groupe Imagerie et Modélisation, Institut Pasteur, Groupe Imagerie et Modélisation; Centre National de la Recherche Scientifique,

    Ricardo Henriques, Mickael Lelek & Christophe Zimmer

  3. Centre National de la Recherche Scientifique, Unité de Recherche Associée 2582, Paris, France

    Ricardo Henriques, Mickael Lelek & Christophe Zimmer

  4. Istituto Scientifico San Raffaele, Università Vita-Salute, Milano, Italia

    Eugenio F Fornasiero & Flavia Valtorta

  5. Unità di Neuroscienze Molecolari, Istituto Italiano di Tecnologia, Milano, Italia

    Eugenio F Fornasiero & Flavia Valtorta

  6. Gene Expression and Biophysics Group, Synthetic Biology Emerging Research Area, Council for Scientific and Industrial Research, Pretoria, South Africa

    Musa M Mhlanga

Authors
  1. Ricardo Henriques
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  2. Mickael Lelek
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  3. Eugenio F Fornasiero
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  4. Flavia Valtorta
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  5. Christophe Zimmer
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  6. Musa M Mhlanga
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Corresponding author

Correspondence to Ricardo Henriques.

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The authors declare no competing financial interests.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–2, Supplementary Methods, Supplementary Note 1, Supplementary Discussion (PDF 903 kb)

Supplementary Software 1

QuickPALM software (ZIP 5714 kb)

Supplementary Software 2

Laser control software (ZIP 5596 kb)

Supplementary Movie 1

A 3D presentation of microtubules shown in Figure 1e,f. The viewing angle is rotated here to show different perspectives. Scale bar, 1,000 nm. (MOV 1376 kb)

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Henriques, R., Lelek, M., Fornasiero, E. et al. QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ. Nat Methods 7, 339–340 (2010). https://doi.org/10.1038/nmeth0510-339

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