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Lifeact mice for studying F-actin dynamics

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Figure 1: Characterization of transgenic Lifeact mice.

References

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Acknowledgements

This work was supported by the Max Planck society.

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Authors and Affiliations

  1. Max Planck Institute of Biochemistry, Max Planck Research Group Cellular Dynamics and Cell Patterning, Martinsried, Germany

    Julia Riedl, Gisela Beck & Roland Wedlich-Söldner

  2. Max Planck Institute of Neurobiology, Max Planck Research Group Axonal Growth and Regeneration, Martinsried, Germany

    Kevin C Flynn & Frank Bradke

  3. Department of Molecular Medicine, Max Planck Institute of Biochemistry, Martinsried, Germany

    Aurelia Raducanu, Michael Bösl & Attila Aszodi

  4. German Heart Centre, Technical University Munich, Munich, Germany

    Florian Gärtner & Steffen Massberg

  5. Max Planck Institute of Biochemistry, Hofschneider Group Leukocyte Migration, Martinsried, Germany

    Michael Sixt

Authors
  1. Julia Riedl
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  2. Kevin C Flynn
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  3. Aurelia Raducanu
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  4. Florian Gärtner
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  5. Gisela Beck
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  6. Michael Bösl
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  7. Frank Bradke
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  8. Steffen Massberg
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  9. Attila Aszodi
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  10. Michael Sixt
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  11. Roland Wedlich-Söldner
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Corresponding authors

Correspondence to Michael Sixt or Roland Wedlich-Söldner.

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Competing interests

J.R., M.S. and R.W.-S. have submitted a patent application (07 023 084.2; Peptide for determining actin structures in living cells) to the EU patent office.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–6, Supplementary Methods (PDF 1847 kb)

Supplementary Video 1

Migrating T-cell expressing Lifeact-EGFP. Images were acquired by TIRFM. Scale bar, 3 μm. (MOV 308 kb)

Supplementary Video 2

Spreading platelet expressing Lifeact-EGFP. Images were acquired by TIRFM. Scale bar, 3 μm. (MOV 58 kb)

Supplementary Video 3

Dividing chondrocytes. Images of tibial cartilage slice cultures were acquired on a spinning disk microscope. Scale bar, 20 μm. (MOV 364 kb)

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Riedl, J., Flynn, K., Raducanu, A. et al. Lifeact mice for studying F-actin dynamics. Nat Methods 7, 168–169 (2010). https://doi.org/10.1038/nmeth0310-168

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