Abstract
A quantitative high-throughput FRET-based method of screening fluorescent protein fusion libraries brings the promise of more detailed interactome maps.
References
Nguyen, A.W. & Daugherty, P.S. Evolutionary optimization of fluorescent proteins for intracellular FRET. Nat. Biotechnol. 23, 355–360 (2005).
You, X. et al. Intracellular protein interaction mapping with FRET hybrids. Proc. Natl. Acad. Sci. USA 103, 18458–18463 (2006).
Rights and permissions
About this article
Cite this article
Kaganman, I. FRETting for a more detailed interactome. Nat Methods 4, 112–113 (2007). https://doi.org/10.1038/nmeth0207-112b
Issue Date:
DOI: https://doi.org/10.1038/nmeth0207-112b
- Springer Nature America, Inc.