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Primer-initiated sequence synthesis to detect and assemble structural variants

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Figure 1: PrInSeS-G principle and validation.

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Acknowledgements

We thank the members of the Lausanne DNA array facility for performing the Illumina sequencing, N. Gheldof for help with the figures, J. Sacchettini for providing the M. tuberculosis H37Rv-JS read data, and the members of the Deplancke laboratory for critical reading of the manuscript. A substantial part of the data analysis was performed at the Vital-IT Center for high-performance computing of the Swiss Institute of Bioinformatics. This work was supported by funds from the Swiss National Science Foundation, Systems X, a Marie Curie International Reintegration grant (B.D.) and the Seventh Research Framework Programme, and by Institutional support from the Ecole Polytechnique Fédérale de Lausanne.

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Author notes

  1. Andreas Massouras and Korneel Hens: These authors contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Systems Biology and Genetics, Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland

    Andreas Massouras, Korneel Hens, Carine Gubelmann & Bart Deplancke

  2. Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland

    Swapna Uplekar & Stewart T Cole

  3. Genohm, Zwijnaarde, Belgium

    Frederik Decouttere

  4. School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne and Swiss Institute of Bioinformatics, Lausanne, Switzerland

    Jacques Rougemont

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  1. Andreas Massouras
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  2. Korneel Hens
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  3. Carine Gubelmann
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  4. Swapna Uplekar
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  5. Frederik Decouttere
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  6. Jacques Rougemont
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  7. Stewart T Cole
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  8. Bart Deplancke
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Corresponding author

Correspondence to Bart Deplancke.

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The authors declare no competing financial interests.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–6, Supplementary Tables 1–5, Supplementary Data, Supplementary Methods (PDF 666 kb)

Supplementary Table 6

List of all consensus Drosophila melanogaster RAL-304 variants. (XLS 23389 kb)

Supplementary Table 7

List of broad sense heritability values for genes with or without sequence variants. (XLS 1581 kb)

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Massouras, A., Hens, K., Gubelmann, C. et al. Primer-initiated sequence synthesis to detect and assemble structural variants. Nat Methods 7, 485–486 (2010). https://doi.org/10.1038/nmeth.f.308

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