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Biochemistry

CRISPR kinetics

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In vitro Cas9-binding assays show the effects of mutations in the target sequence on binding kinetics.

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References

  1. Boyle, E.A. et al. High-throughput biochemical profiling reveals sequence determinants of dCas9 off-target binding and unbinding. Proc. Nat. Acad. Sci. U.S.A. 114, 5461–5466 (2017).

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Authors
  1. Nicole Rusk
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Rusk, N. CRISPR kinetics. Nat Methods 14, 650–651 (2017). https://doi.org/10.1038/nmeth.4360

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  • DOI: https://doi.org/10.1038/nmeth.4360

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