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The NIH Protein Capture Reagents Program (PCRP): a standardized protein affinity reagent toolbox

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Figure 1: Flowchart showing pipeline for rAb and mAb generation and validation.

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Acknowledgements

This work was supported by NIH grants U54HG006434, U01DC011485, and U54HG006436. The authors wish to sincerely thank the following individuals who have provided helpful and critical input into the production program throughout the past five years: A. Kossiakoff, J. Wells, S. Koide, S. Sidhu, S. Miersch, M. Paduch, M. Hornsby, and A. Saaf (Recombinant Antibody Network); J. Boeke, P. Mita, E. Albino, Z. Rivera-Pacheco, and P. Ramos (JHU/CDI); Jessica J. Smith (NIH OD Common Fund); J.S. Trimmer, M.J. Taussig (cochairs), W. Marasco, J. Scott, and G. Georgiou (PCRP External Scientific Panel); N. Starner, G. Halusa, R. Guthridge, G. Whiteley, and R. Roberts (Leidos Biomedical Research); C. Fletcher-Hoppe, T. Hiltke, C. Edmonds, and A. Felsenfeld (PCRP team); G. Montelione (Rutgers University); and R.M. Myers and M. Mackiewicz (HudsonAlpha). The opinions expressed in this article do not necessarily reflect the views of the National Institutes of Health or of the Department of Health and Human Services.

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Authors and Affiliations

  1. Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, Baltimore, Maryland, USA

    Seth Blackshaw & Anand Venkataraman

  2. Department of Neurology, Johns Hopkins University, Baltimore, Maryland, USA

    Seth Blackshaw

  3. Department of Ophthalmology, Johns Hopkins University, Baltimore, Maryland, USA

    Seth Blackshaw

  4. Center for Human Systems Biology, Johns Hopkins University, Baltimore, Maryland, USA

    Seth Blackshaw, Heng Zhu & Joel S Bader

  5. Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    Seth Blackshaw

  6. CDI Laboratories, Guanajibo Research and Innovation Park, Mayaguez, Puerto Rico

    Jose Irizarry & Ignacio Pino

  7. Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA

    Kun Yang

  8. Department of Molecular Biology and Biochemistry, Rutgers University, New Brunswick, New Jersey, USA

    Stephen Anderson & Elliot Campbell

  9. Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey, USA

    Stephen Anderson & Elliot Campbell

  10. National Human Genome Research Institute, Bethesda, Maryland, USA

    Christine L Gatlin

  11. National Institutes of Health, Bethesda, Maryland, USA

    Christine L Gatlin, Nancy L Freeman, Ravi Basavappa & Randall Stewart

  12. Division of Scientific Programs, National Institute on Deafness and Other Communications Disorders, Bethesda, Maryland, USA

    Nancy L Freeman

  13. Office of Strategic Coordination, Office of the Director, National Institutes of Health, Bethesda, Maryland, USA

    Ravi Basavappa

  14. National Institute of Neurological Disorders and Stroke, Bethesda, Maryland, USA

    Randall Stewart

  15. Advanced Biomedical Computing Center, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc., Frederick, Maryland, USA

    Michael A Loss

  16. Department of Pharmacology, Johns Hopkins University, Baltimore, Maryland, USA

    Heng Zhu

  17. Neuroscience Center, Bethesda, Maryland, USA

    Joel S Bader

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  1. Seth Blackshaw
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  2. Anand Venkataraman
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  3. Jose Irizarry
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  7. Christine L Gatlin
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  8. Nancy L Freeman
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  11. Michael A Loss
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  12. Ignacio Pino
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  13. Heng Zhu
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  14. Joel S Bader
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Corresponding author

Correspondence to Seth Blackshaw.

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Competing interests

S.B., H.Z., and I.P. are cofounders and shareholders of CDI Labs Inc.; J.S.B. is a consultant to CDI.

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Blackshaw, S., Venkataraman, A., Irizarry, J. et al. The NIH Protein Capture Reagents Program (PCRP): a standardized protein affinity reagent toolbox. Nat Methods 13, 805–806 (2016). https://doi.org/10.1038/nmeth.4013

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