By removing modified nucleotides that block reverse transcriptase, two methods have now made tRNAs amenable to RNA-seq.
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Acknowledgements
J.E.W. is supported by US National Institutes of Health grant R00-GM104166 and the Rita Allen Foundation.
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Wilusz, J. Removing roadblocks to deep sequencing of modified RNAs. Nat Methods 12, 821–822 (2015). https://doi.org/10.1038/nmeth.3516
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DOI: https://doi.org/10.1038/nmeth.3516
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