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Light-sheet imaging for systems neuroscience

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Developments in electrical and optical recording technology are scaling up the size of neuronal populations that can be monitored simultaneously. Light-sheet imaging is rapidly gaining traction as a method for optically interrogating activity in large networks and presents both opportunities and challenges for understanding circuit function.

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Figure 1: The ultramicroscope, the first light-sheet microscope.
Figure 2: Modern light-sheet microscopy in systems neuroscience.

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Authors and Affiliations

  1. Philipp J. Keller, Misha B. Ahrens and Jeremy Freeman are at Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.,

    Philipp J Keller, Misha B Ahrens & Jeremy Freeman

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  1. Philipp J Keller
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  2. Misha B Ahrens
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  3. Jeremy Freeman
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Correspondence to Philipp J Keller, Misha B Ahrens or Jeremy Freeman.

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Keller, P., Ahrens, M. & Freeman, J. Light-sheet imaging for systems neuroscience. Nat Methods 12, 27–29 (2015). https://doi.org/10.1038/nmeth.3214

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