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Prevention of overfitting in cryo-EM structure determination

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Figure 1: The prevention of overfitting.

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Acknowledgements

We are grateful to T. Crowther and R. Henderson for helpful discussions and to J. Grimmett for help with computing. T. Crowther provided hepatitis B data, and the National Center for Macromolecular Imaging, which is funded by US National Institutes of Health grant P41RR02250, provided GroEL data. This work was funded by the UK Medical Research Council through grant MC_UP_A025_1013 to S.H.W.S.

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Authors and Affiliations

  1. Medical Research Council Laboratory of Molecular Biology, Cambridge, UK

    Sjors H W Scheres & Shaoxia Chen

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  1. Sjors H W Scheres
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  2. Shaoxia Chen
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Correspondence to Sjors H W Scheres.

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The authors declare no competing financial interests.

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Supplementary Figures 1–4, Supplementary Table 1, Supplementary Methods and Supplementary Software (PDF 765 kb)

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Scheres, S., Chen, S. Prevention of overfitting in cryo-EM structure determination. Nat Methods 9, 853–854 (2012). https://doi.org/10.1038/nmeth.2115

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