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Protease bias in absolute protein quantitation

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Figure 1: Comparison of absolute protein quantification by spectral counts with different enzymes.

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Acknowledgements

This work was supported by the PRIME-XS project, grant agreement 262067, funded by the European Union 7th Framework Programme; the Netherlands Proteomics Centre of the Netherlands Genomics Initiative; the Centre for Biomedical Genetics; the Netherlands Organization for Scientific Research (NWO) VIDI grant (700.10.429) and the Netherlands Bioinformatics Centre.

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Authors and Affiliations

  1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands

    Mao Peng, Nadia Taouatas, Salvatore Cappadona, Bas van Breukelen, Shabaz Mohammed, Arjen Scholten & Albert J R Heck

  2. Netherlands Proteomics Centre, Utrecht, The Netherlands

    Mao Peng, Nadia Taouatas, Salvatore Cappadona, Bas van Breukelen, Shabaz Mohammed, Arjen Scholten & Albert J R Heck

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  1. Mao Peng
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  2. Nadia Taouatas
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  3. Salvatore Cappadona
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  4. Bas van Breukelen
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  6. Arjen Scholten
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  7. Albert J R Heck
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Corresponding authors

Correspondence to Arjen Scholten or Albert J R Heck.

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Supplementary Figure 1-3, Supplementary Table 4 and Supplementary Methods (PDF 1333 kb)

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Supplementary Table 1 (XLSX 2336 kb)

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Supplementary Table 2 (XLSX 3154 kb)

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Supplementary Table 3 (XLSX 1338 kb)

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Peng, M., Taouatas, N., Cappadona, S. et al. Protease bias in absolute protein quantitation. Nat Methods 9, 524–525 (2012). https://doi.org/10.1038/nmeth.2031

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