Skip to main content
Log in

Zinc fingers hit off target

  • Community Corner
  • Published:

From Nature Medicine

View current issue Submit your manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Pattanayak, V., Ramirez, C.L., Joung, J.K. & Liu, D.R. Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection. Nat. Meth. 8, 765–770 (2011).

    Article  CAS  Google Scholar 

  2. Gabriel, R. et al. An unbiased genome-wide analysis of zinc-finger nuclease specificity. Nat. Biotechnol. 29, 816–823 (2011).

    Article  CAS  Google Scholar 

  3. Hockemeyer, D. et al. Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat. Biotechnol. 27, 851–857 (2009).

    Article  CAS  Google Scholar 

  4. Zou, J. et al. Oxidase deficient neutrophils from X-linked chronic granulomatous disease iPS cells: functional correction by zinc finger nuclease mediated safe harbor targeting. Blood 117, 5561–5572 (2011).

    Article  CAS  Google Scholar 

  5. ENCODE Project Consortium et al. Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project. Nature 447, 799–816 (2007).

  6. Li, H. et al. In vivo genome editing restores haemostasis in a mouse model of haemophilia. Nature 475, 217–221 (2011).

    Article  CAS  Google Scholar 

  7. Porteus, M.H. & Baltimore, D. Chimeric nucleases stimulate gene targeting in human cells. Science 300, 763 (2003).

    Article  Google Scholar 

  8. Pruett-Miller, S.M., Reading, D.W., Porter, S.N. & Porteus, M.H. Attenuation of zinc finger nuclease toxicity by small-molecule regulation of protein levels. PLoS Genet. 5, e1000376 (2009).

    Article  Google Scholar 

Download references

Ethics declarations

Competing interests

K.H. is a co-inventor on a pending patent using zinc finger nucleases to do genome editing in hemophilia.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zinc fingers hit off target. Nat Med 17, 1192–1193 (2011). https://doi.org/10.1038/nm1011-1192

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nm1011-1192

  • Springer Nature America, Inc.

This article is cited by

Navigation