This is a preview of subscription content, log in via an institution to check access.
References
Sanger, F., Nicklen, S. & Coulson, A.R. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463–5467 (1977).
Smith, L.M. et al. Fluorescence detection in automated DNA sequence analysis. Nature 321, 674–679 (1986).
Prober, J.M. et al. A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides. Science 238, 336–341 (1987).
Connell, C.R. et al. Automated DNA sequence analysis. BioTechniques 5, 342–348 (1987).
Mathies, R.A. & Huang, X.C. Capillary array electrophoresis: An approach to high-speed, high-throughput DNA sequencing. Nature 359, 167–169 (1992).
Tabor, S. & Richardson, C.C. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc. Natl. Acad. Sci. USA 84, 4767–4771 (1987).
Tabor, S. & Richardson, C.C. A single residue in DNA polymerases of the Escherichia coliDNA polymerase I family is critical for distinguishing between deoxy- and dideoxyribonucleotides. Proc. Natl. Acad. Sci. USA 92, 6339–6343 (1995).
Ju, J., Ruan, C., Fuller, C.W., Glazer, A.N. & Mathies, R.A. Fluorescence energy transfer dye-labeled primers for DNA sequencing and analysis. Proc. Natl. Acad. Sci. USA 92, 4347–4351 (1995).
Ju, J. et al. Design and synthesis of fluorescence energy transfer dye-labeled primers and their application for DNA sequencing and analysis. Anal. Biochem. 231, 131–140 (1995).
Wang, Y., Ju, J., Carpenter, B.A., Atherton, J.M., Sensabaugh, G.F. & Mathies, R.A. Rapid sizing of short tandem repeat alleles using capillary array electrophoresis and energy-transfer fluorescent primers. Anal. Chetn. 67, 1197–1203 (1995).
Förster, T. in Modem Quantum Chemistry, Istanbul Lectures, Part III (ed. Sinanoglu, O.) 93–137 (Academic Press, New York and London, 1965).
Stryer, L. Fluorescence energy transfer as a spectro-scopic ruler. Annu. Rev. Biochem. 47, 819–846 (1978).
Glazer, A.N. & Stryer, L. Fluorescent tandem phycobiliprotein conjugates. Biophys. J. 43, 383–386 (1983).
Benson, S.C., Mathies, R.A. & Glazer, A.N., Heterodimeric DNA-binding dyes designed for energy transfer: stability and applications of the DNA complexes. Nucleic Acids Res. 21, 5720–5726 (1993).
Ju, J., Glazer, A.N. & Mathies, R.A. Cassette labeling for facile construction of energy transfer fluorescent primers. Nucleic Acids Res. (in the press).
Reeve, M.A. & Fuller, C.W. A novel thermostable polymerase for DNA sequencing. Nature 376, 796–797 (1995).
Hammond, H.A., Jin, L., Zhong, Y., Caskey, C.T. & Chakraborty, R. Evaluation of 13 short tandem repeat loci for use in personal identification applications. Am. J. Hum. Genet. 55, 175–189 (1994).
Pease, A.C. et al. Light-generated oligonucleotide arrays for rapid DNA sequence analysis. Proc. Natl. Acad. Sci. USA 91, 5022–5026 (1994).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ju, J., Glazer, A. & Mathies, R. Energy transfer primers: A new fluorescence labeling paradigm for DNA sequencing and analysis. Nat Med 2, 246–249 (1996). https://doi.org/10.1038/nm0296-246
Issue Date:
DOI: https://doi.org/10.1038/nm0296-246
- Springer Nature America, Inc.
This article is cited by
-
Single nucleotide polymorphism genotyping: biochemistry, protocol, cost and throughput
The Pharmacogenomics Journal (2003)
-
An economic method for the fluorescent labeling of PCR fragments
Nature Biotechnology (2000)
-
Synthesis and fluorescent properties of 5-(1-pyrenylethynyl)-2′-deoxyuridine-containing oligodeoxynucleotides
Russian Journal of Bioorganic Chemistry (2000)