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NGS library preparation may generate artifactual integration sites of AAV vectors

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Figure 1: Distribution of junction breakpoints along rAAV genomes.

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Acknowledgements

This study has been supported by funds from the Association Française contre les Myopathies and the French Regional Council of Pays-de-la-Loire. We thank the genomics and bioinformatics core facilities of Nantes (Biogenouest) for their expert services. We are also grateful to M. Haskins for his critical reading.

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Authors and Affiliations

  1. INSERM, UMR 1089, Nantes, France

    Benjamin Cogné, Richard Snyder, Jean-Baptiste Dupont, Philippe Moullier & Adrien Leger

  2. University of Nantes, Nantes, France

    Benjamin Cogné, Pierre Lindenbaum, Jean-Baptiste Dupont, Richard Redon, Philippe Moullier & Adrien Leger

  3. Nantes University Hospital, Nantes, France

    Benjamin Cogné, Pierre Lindenbaum, Jean-Baptiste Dupont, Richard Redon, Philippe Moullier & Adrien Leger

  4. Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, Florida, USA

    Richard Snyder & Philippe Moullier

  5. Center of Excellence for Regenerative Health Biotechnology, University of Florida, Alachua, Florida, USA

    Richard Snyder

  6. INSERM, UMR 1087, L'Institut du Thorax, Nantes, France

    Pierre Lindenbaum & Richard Redon

  7. CNRS, UMR 6291, Nantes, France

    Pierre Lindenbaum & Richard Redon

Authors
  1. Benjamin Cogné
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  2. Richard Snyder
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  3. Pierre Lindenbaum
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  4. Jean-Baptiste Dupont
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  5. Richard Redon
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  6. Philippe Moullier
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  7. Adrien Leger
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Corresponding author

Correspondence to Adrien Leger.

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Competing interests

R.S. is an inventor on patents related to recombinant AAV and owns equity in a gene therapy company, AGCT, that is commercializing AAV for gene therapy applications.

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Supplementary Methods, Supplementary Table 1 and Supplementary Figures 1–3 (PDF 342 kb)

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Cogné, B., Snyder, R., Lindenbaum, P. et al. NGS library preparation may generate artifactual integration sites of AAV vectors. Nat Med 20, 577–578 (2014). https://doi.org/10.1038/nm.3578

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