By identifying gene products whose knockdown is associated with phenotypic changes, large-scale RNA-mediated interference screens have demonstrated previously unknown components of biological pathways. This commentary provides general guidelines for using such screens to answer questions of immunological interest.
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Acknowledgements
Supported by the US National Institutes of Health (A.R.), the Juvenile Diabetes Research Foundation (A.R.), the Canadian Institutes for Health Research (S.S.) and the Leukemia and Lymphoma Society (S.S.).
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A.R. is a shareholder of and scientific advisor to CaliMedica and receives laboratory support from Glaxo-Smith-Kline.
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Sharma, S., Rao, A. RNAi screening: tips and techniques. Nat Immunol 10, 799–804 (2009). https://doi.org/10.1038/ni0809-799
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DOI: https://doi.org/10.1038/ni0809-799
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