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Response to 'Autoantibodies to GPI and creatine kinase in RA' and 'Few human autoimmune sera detect GPI'

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Figure 1: Correlation between serum IgG reactivity to purified rabbit GPI and recombinant human GPI.

References

  1. Matsumoto, I., Staub, A., Benoist, C. & Mathis, D. Science 286, 1732–1735 (1999).

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  2. Schaller, M., Burton, D. R. & Ditzel, H. J. Nature Immunol. 2, 746–753 (2001).

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Authors and Affiliations

  1. Departments of Immunology, IMM2, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, 92037, CA, USA

    Monica Schaller, Vivian M. Benoit & Henrik J. Ditzel

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  1. Monica Schaller
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  2. Vivian M. Benoit
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  3. Henrik J. Ditzel
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Note: Supplementary information is available on the Nature Immunology website.

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Web Figure 1.

Silver-stained gel of human recombinant GPI purified using glutathione sepharose 4B following site-specific protease cleavage of GST-GPI fusion protein with thrombin. The GST-GPI was expressed in Escherichia coli BL21 using the pGEX-4T-1 vector (Amersham). Lane 1: Human recombinant GPI (5 µg), lane 2: Low range silver-stain standard (Biorad). (JPG 6 kb)

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Schaller, M., Benoit, V. & Ditzel, H. Response to 'Autoantibodies to GPI and creatine kinase in RA' and 'Few human autoimmune sera detect GPI'. Nat Immunol 3, 412–413 (2002). https://doi.org/10.1038/ni0502-412

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