Skip to main content

Advertisement

SpringerLink
Log in

Mass cytometry: blessed with the curse of dimensionality

  • Comment
  • Published:

From Nature Immunology

View current issue Submit your manuscript

Abstract

Immunologists are being compelled to develop new high-dimensional perspectives of cellular heterogeneity and to determine which applications best exploit the power of mass cytometry and associated multiplex approaches.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: The curse of dimensionality.
Figure 2: The emergence of high-resolution cellular phenotypes through nonlinear summation of parameters.

References

  1. Robinson, J.P. & Roederer, M. Science 350, 739–740 (2015).

    Article  CAS  Google Scholar 

  2. Bandura, D.R. et al. Anal. Chem. 81, 6813–6822 (2009).

    Article  CAS  Google Scholar 

  3. Bendall, S.C. et al. Science 332, 687–696 (2011).

    Article  CAS  Google Scholar 

  4. Newell, E.W., Sigal, N., Bendall, S.C., Nolan, G.P. & Davis, M.M. Immunity 36, 142–152 (2012).

    Article  CAS  Google Scholar 

  5. Bendall, S.C., Nolan, G.P., Roederer, M. & Chattopadhyay, P.K. Trends Immunol. 33, 323–332 (2012).

    Article  CAS  Google Scholar 

  6. Chattopadhyay, P.K., Gierahn, T.M., Roederer, M. & Love, J.C. Nat. Immunol. 15, 128–135 (2014).

    Article  CAS  Google Scholar 

  7. Leipold, M.D., Newell, E.W. & Maecker, H.T. Methods Mol. Biol. 1343, 81–95 (2015).

    Article  CAS  Google Scholar 

  8. Perfetto, S.P., Chattopadhyay, P.K. & Roederer, M. Nat. Rev. Immunol. 4, 648–655 (2004).

    Article  CAS  Google Scholar 

  9. Bodenmiller, B. et al. Nat. Biotechnol. 30, 858–867 (2012).

    Article  CAS  Google Scholar 

  10. Newell, E.W. & Davis, M.M. Nat. Biotechnol. 32, 149–157 (2014).

    Article  CAS  Google Scholar 

  11. Newell, E.W. et al. Nat. Biotechnol. 31, 623–629 (2013).

    Article  CAS  Google Scholar 

  12. Bellman, R. Dynamic Programming 1st edn. (Princeton University Press, 1957).

    Google Scholar 

  13. Aghaeepour, N. et al. Nat. Methods 10, 228–238 (2013).

    Article  CAS  Google Scholar 

  14. Mair, F. et al. Eur. J. Immunol. 46, 34–43 (2016).

    Article  CAS  Google Scholar 

  15. Diggins, K.E., Ferrell, P.B. Jr. & Irish, J.M. Methods 82, 55–63 (2015).

    Article  CAS  Google Scholar 

  16. Zunder, E.R., Lujan, E., Goltsev, Y., Wernig, M. & Nolan, G.P. Cell Stem Cell 16, 323–337 (2015).

    Article  CAS  Google Scholar 

  17. Spitzer, M.H. et al. Science 349, 1259425 (2015).

    Article  Google Scholar 

  18. Haghverdi, L., Buettner, F. & Theis, F.J. Bioinformatics 31, 2989–2998 (2015).

    Article  CAS  Google Scholar 

  19. Tenenbaum, J.B., de Silva, V. & Langford, J.C. Science 290, 2319–2323 (2000).

    Article  CAS  Google Scholar 

  20. van der Maaten, L. & Hinton, G. J. Mach. Learn. Res. 9, 85 (2008).

    Google Scholar 

  21. Becher, B. et al. Nat. Immunol. 15, 1181–1189 (2014).

    Article  CAS  Google Scholar 

  22. Cheng, Y., Wong, M.T., van der Maaten, L. & Newell, E.W. J. Immunol. 196, 924–932 (2016).

    Article  CAS  Google Scholar 

  23. Ginhoux, F., Schultze, J.L., Murray, P.J., Ochando, J. & Biswas, S.K. Nat. Immunol. 17, 34–40 (2016).

    Article  CAS  Google Scholar 

  24. Gaudillière, B. et al. Sci. Transl. Med. 6, 255ra131 (2014).

    Article  Google Scholar 

  25. Wong, M.T. et al. Cell Rep. 11, 1822–1833 (2015).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank all members of the Newell laboratory, especially M. Fehlings and Y. Simoni, for comments and suggestions, and previous and ongoing collaborators for data that have influenced the opinions and general observations here. Supported by A-STAR/SIgN core funding and the ASTAR/SIgN immunomonitoring platform (E.W.N.).

Author information

Authors and Affiliations

  1. Evan W. Newell and Yang Cheng are with the Singapore Immunology Network, Agency for Science Technology and Research, Singapore, and the Nanyang Technological University School of Biological Sciences, Singapore.,

    Evan W Newell & Yang Cheng

Authors
  1. Evan W Newell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yang Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Evan W Newell.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Newell, E., Cheng, Y. Mass cytometry: blessed with the curse of dimensionality. Nat Immunol 17, 890–895 (2016). https://doi.org/10.1038/ni.3485

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/ni.3485

  • Springer Nature America, Inc.

This article is cited by

Search

Navigation