Abstract
Skin-derived dendritic cells (DCs) include Langerhans cells, classical dermal DCs and a langerin-positive CD103+ dermal subset. We examined their involvement in the presentation of skin-associated viral and self antigens. Only the CD103+ subset efficiently presented antigens of herpes simplex virus type 1 to naive CD8+ T cells, although all subsets presented these antigens to CD4+ T cells. This showed that CD103+ DCs were the migratory subset most efficient at processing viral antigens into the major histocompatibility complex class I pathway, potentially through cross-presentation. This was supported by data showing only CD103+ DCs efficiently cross-presented skin-derived self antigens. This indicates CD103+ DCs are the main migratory subtype able to cross-present viral and self antigens, which identifies another level of specialization for skin DCs.
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Acknowledgements
We thank the flow cytometry facilities and animal facility staff of the Walter and Eliza Hall Institute of Medical Research and K. Field; we also thank B. Davies and J. Langley for technical assistance. Supported by Deutsche Forschungsgemeinschaft (BE 3285-1/2 to S.B.), the National Health and Medical Research Council of Australia (W.R.H., F.R.C., A.G.B. and P.G.W.) and the Howard Hughes Medical Institute (W.R.H.).
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Bedoui, S., Whitney, P., Waithman, J. et al. Cross-presentation of viral and self antigens by skin-derived CD103+ dendritic cells. Nat Immunol 10, 488–495 (2009). https://doi.org/10.1038/ni.1724
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DOI: https://doi.org/10.1038/ni.1724
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