Silencing gene expression by RNA interference (RNAi) has become a daily activity for mammalian cell biologists. Those who often use this approach have come to appreciate its occasional frustrations and potential limitations. A pair of recent papers in Cell provides rules for designing short interfering RNAs (siRNAs) that are both validated at the practical level and understood mechanistically.
References
Hannon, G.J. Nature 418, 244–251 (2002).
Nykanen, A., Haley, B. & Zamore, P.D. Cell 107, 309–321 (2001).
Schwarz, D.S., Hutvagner, G., Haley, B. & Zamore, P.D. Mol. Cell 10, 537–548 (2002).
Martinez, J., Patkaniowska, A., Urlaub, H., Luhrmann, R. & Tuschl, T. Cell 110, 563–574 (2002).
Elbashir, S.M. et al. Nature 411, 494–498 (2001).
Caplen, N.J., Parrish, S., Imani, F., Fire, A. & Morgan, R.A. Proc. Natl. Acad. Sci. USA 98, 9742–9747 (2001).
Khvorova, A., Reynolds, A. & Jayasena, S.D. Cell 115, 209–216 (2003).
Schwarz, D.S. et al. Cell 115, 199–208 (2003).
Lee, Y. et al. Nature 425, 415–419 (2003).
Turner, D.H. Curr. Opin. Struct. Biol. 6, 299–304 (1996).
Griffiths-Jones, S., Bateman, A., Marshall, M., Khanna, A. & Eddy, S.R. Nucleic Acids Res. 31, 439–441 (2003).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Silva, J., Sachidanandam, R. & Hannon, G. Free energy lights the path toward more effective RNAi. Nat Genet 35, 303–305 (2003). https://doi.org/10.1038/ng1203-303
Issue Date:
DOI: https://doi.org/10.1038/ng1203-303
- Springer Nature America, Inc.
This article is cited by
-
Targeted screening of SiRNA directed HBV polymerase gene for effective inhibition of HBV expression
Journal of Huazhong University of Science and Technology [Medical Sciences] (2008)
-
Germ line transmission and expression of an RNAi cassette in mice generated by a lentiviral vector system
Transgenic Research (2007)
-
Computational models with thermodynamic and composition features improve siRNA design
BMC Bioinformatics (2006)
-
Inhibition of gene expression in mice muscle by in vivo electrically mediated siRNA delivery
Gene Therapy (2005)
-
Unlocking the potential of the human genome with RNA interference
Nature (2004)