Figure 2: Nano-Pd mediated catalysis in live cell culture.
From: Nano-palladium is a cellular catalyst for in vivo chemistry
(a) Scheme for synthesis and nanoencapsulation of Pd-NP. (b) TEM shows spherical Pd-NP 50 nm in diameter (scale bar, 100 nm). (c) HT1080 cells were treated with 25 μM Pd-NP, 30 μM Alloc2R110 or the combination for 24 h, and intracellular fluorescence from uncaged R110 was quantified after repeated washing by fluorescence microscopy (*P<10−4; two-tailed Student’s t-test, n=16). (d) Formation of a coumarin fluorophore (7-diethylaminocoumarin) from its non-fluorescent precursor via intramolecular Heck coupling was monitored in HT1080 cells, which were treated with 12.5 μM Pd-NP, 30 μM coumarin precursor or the combination for 24 h. Intracellular fluorescence was imaged after repeated washing, and found to be significantly brighter with the combination compared to either treatment alone (P=0.002; Student’s two-tailed t-test, n=18). Scale bar, 100 μm.