Skip to main content

Advertisement

SpringerLink
Log in

Reply: reducing background fluorescence reveals adhesions in 3D matrices

  • Correspondence
  • Published:

From Nature Cell Biology

View current issue Submit your manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: Focal adhesion visualization in live HT-1080 cells.
Figure 2: Properties of cells on or near the bottom versus fully embedded in 3D collagen gel.

References

  1. Fraley, S. I. et al. Nat. Cell Biol. 12, 598–604 (2010).

    Article  CAS  Google Scholar 

  2. Watanabe, N. & Mitchison, T. J. Science 295, 1083–1086 (2002).

    Article  CAS  Google Scholar 

  3. Provenzano, P. P., Inman, D. R., Eliceiri, K. W. & Keely, P. J. Oncogene 28, 4326–4343 (2009).

    Article  CAS  Google Scholar 

  4. Wozniak, M. A., Desai, R., Solski, P. A., Der, C. J. & Keely, P. J. J. Cell Biol. 163, 583–595 (2003).

    Article  CAS  Google Scholar 

  5. Pelham, R. J., Jr. & Wang, Y. Proc. Natl Acad. Sci. USA 94, 13661–13665 (1997).

    Article  CAS  Google Scholar 

  6. Raub, C. B. et al. Biophys. J. 92, 2212–2222 (2007).

    Article  CAS  Google Scholar 

  7. Sabeh, F., Shimizu-Hirota, R. & Weiss, S. J. J. Cell Biol. 185, 11–19 (2009).

    Article  CAS  Google Scholar 

  8. Pratt, S. J., Epple, H., Ward, M., Feng, Y., Braga, V. M. & Longmore, G. D. J. Cell Biol. 168, 813–824 (2005).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by NIH grants CA143868, GM084204, GM080673 and CA85839 to G.D.L. and D.W. S.I.F. was supported by ARCS and NSF-GRFP.

Author information

Authors and Affiliations

  1. Departments of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, 21218, MD, USA

    Stephanie I. Fraley & Denis Wirtz

  2. Johns Hopkins Physical Sciences in Oncology Centre, Johns Hopkins University, Baltimore, 21218, MD, USA

    Stephanie I. Fraley, Yunfeng Feng, Denis Wirtz & Gregory D. Longmore

  3. Departments of Medicine and Cell Biology and Physiology, Washington University School of Medicine, St. Louis, 63110, MO, USA

    Yunfeng Feng & Gregory D. Longmore

  4. BRIGHT Institute, Washington University School of Medicine, St. Louis, 63110, MO, USA

    Yunfeng Feng & Gregory D. Longmore

Authors
  1. Stephanie I. Fraley
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yunfeng Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Denis Wirtz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gregory D. Longmore
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Y. F. generated YFP-paxillin and GFP-zyxin constructs. S. F. performed all experiments and analysis and co-wrote the manuscript; G. L. and D. W. co-supervised the project and co-wrote the manuscript.

Corresponding authors

Correspondence to Denis Wirtz or Gregory D. Longmore.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fraley, S., Feng, Y., Wirtz, D. et al. Reply: reducing background fluorescence reveals adhesions in 3D matrices. Nat Cell Biol 13, 5–7 (2011). https://doi.org/10.1038/ncb0111-5

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/ncb0111-5

  • Springer Nature Limited

This article is cited by

Search

Navigation