Abstract
A recombinant cauliflower mosaic virus (Ca-MTII) was constructed by inserting a cDNA clone of Chinese hamster metallothionein II into the open reading frame II of the cloned virus pCa-BB1. Systemically-infected Brassica campestris tissue contained metallothionein at a level of 0.5 percent of the soluble leaf protein. This efficient expression conferred four times the Cd-binding capacity when compared with Ca-BB1 infected leaves. Ca-MTII-infected leaves exposed to 1 mM CdCl2 bound all the free Cd whereas uninfected leaves possessed 43.8 nmol free Cd per milligram of protein. This may be responible for Cd resistance in the Ca-MTII plant cells. Metallothionein is the first mammalian gene product shown to be functional in plants.
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Lefebvre, D., Miki, B. & Laliberté, JF. Mammalian Metallothionein Functions in Plants. Nat Biotechnol 5, 1053–1056 (1987). https://doi.org/10.1038/nbt1087-1053
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DOI: https://doi.org/10.1038/nbt1087-1053
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