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Replacing cRNA targets with cDNA reduces microarray cross-hybridization

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Figure 1: Comparison of microarray data from Jurkat-cell RNA with and without spiked transcripts.

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Authors and Affiliations

  1. Children's Hospital Informatics Program at the Harvard-MIT Division of Health Sciences and Technology (CHIP@HST), Harvard Medical School, Boston, 02115, Massachusetts, USA

    Aron C Eklund & Zoltan Szallasi

  2. Center for Neurologic Diseases, Brigham and Women's Hospital, 65 Landsdowne St., Cambridge, 02139, Massachusetts, USA

    Aron C Eklund

  3. NuGEN Technologies, Inc., 821 Industrial Rd, Unit A, San Carlos, 94070, California, USA

    Leah R Turner, Pengchin Chen, Gianfranco deFeo & Anne R Kopf-Sill

  4. Department of Physics, University of Massachusetts Boston, Boston, 02125, Massachusetts, USA

    Roderick V Jensen

Authors
  1. Aron C Eklund
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  2. Leah R Turner
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  3. Pengchin Chen
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  4. Roderick V Jensen
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  5. Gianfranco deFeo
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  6. Anne R Kopf-Sill
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  7. Zoltan Szallasi
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Eklund, A., Turner, L., Chen, P. et al. Replacing cRNA targets with cDNA reduces microarray cross-hybridization. Nat Biotechnol 24, 1071–1073 (2006). https://doi.org/10.1038/nbt0906-1071

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