Abstract
Enzyme-containing polymeric materials have been developed that have high activity and stability in both aqueous and organic media. These biocatalytic plastics, containing α-chymotrypsin and subtilisin Carlsberg, can contain up to 50% (w/w) total protein in plastic materials such as poly(methyl methacrylate, styrane, vinyl acetate, and ethyl vinyl ether). The activation achieved in organic solvents by incorporating proteases in plastic matrices allows for the efficient synthesis of peptides, and sugar and nucleoside esters. The marriage of enzyme technology with polymer chemistry opens up an array of unique applications for plastic enzymes, including active and stable biocatalysts in paints, coatings, resins, foams, and beads, as well as membranes, fibers, and tubings.
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References
Blanch, H.W. and Clark, D.S. 1995. Biochemical Engineering. Marcel Dekker, New York, NY.
Wiseman, A. 1985. Handbook of Enzyme Biotechnology, 2nd ed., Ellis Harwood, Ltd., New York, NY.
Clark, D.S. 1994. Prospects for exploiting immobilization to modify enzyme activity. Trends. Biotechnol. 12: 439.
Martinek, K., Klibanov, A.M., Goldmacher, V.S. and Berezin, I.V. 1977. The principles of enzyme stabilization I. Increase in thermostability of enzymes covalently bound to a complementary surface of a polymer support in a multipoint fashion. Biochim. Biophys. Acta 485: 1–12.
Dordick, J.S. 1989. Enzymatic catalysis in monophasic organic solvents. Enzyme Microb. Technol. 11: 194–211.
Takahashi, K., Ajima, A., Yoshimoto, T., Okada, M., Matsushima, A., Tamaura, Y. and Inada, Y. 1985. Chemical reactions by polyethylene glycol modified enzymes in chlorinated hydrocarbons. J. Org. Chem. 50: 3414–3415.
Pina, C., Clark, D.S. and Blanch, H.W. 1989. The activity of PEG-modified chymotrypsin in aqueous and organic media. Biotechnol. Blotechniq. 3: 333–338.
Yang, Z., Mesiano, A.J., Venkatasubramanian, S., Gross, S.H., Harris, J.M. and Russell, A.J. 1995. Activity and stability of enzymes incorporated into acrylic polymers. J. Am. Chem. Soc. 117: 4843–4850.
Ito, Y., Fujii, H. and Imanishi, Y. 1993. Catalytic peptide synthesis by trypsin modified with polystyrene in chloroform. Biotechnol. Prog. 9: 128–130.
Ito, Y., Fujii, H. and Imanishi, Y. 1992. Enzyme hybridization with synthetic polymers for use in organic media. Makromol. Chem. Rapid Commun. 13: 315–319.
Paradkar, V.M. and Dordick, J.S. 1994. Aqueous-like activity of α-chymotrypsin dissolved in nearly anhydrous organic solvents. J. Am. Chem. Soc. 116: 5009–5010.
Paradkar, V.M. and Dordick, J.S. 1994. Extraction and solubilization of chymotrypsin into isooctane in the presence of low concentrations of aerosol OT in the absence of reversed micelles. Biotechnol. Bioeng. 43: 529–540.
Matsuura, J., Powers, M.E., Manning, M.C. and Shefter, E. 1993. Structure and stability of insulin dissolved in 1-octanol. J. Am. Chem. Soc. 115: 1261–1264.
Meyer, J.D., Matsuura, J.E., Kendrick, B.S., Evans, E.S., Evans, G.J. and Manning, M.C. 1995. Solution behavior of α-chymotrypsin dissolved in nonpolar organic solvents via hydrophobic ion-pairing. Biopolymers 35: 451–456.
Klibanov, A.M. 1990. Asymmetric transformations catalyzed by enzymes in organic solvent. Ace. Chem. Res. 23: 114–120.
Tramper, J., VermŸe, M.H., Beeftink, H.H. and von Stockar, U. (eds.). 1992. Biocatalysis in Non-cotiventional Media. Elsevier, Amsterdam, The Netherlands.
Odian, G. 1991. Principles of Polymerization. John Wiley & Sons, Inc. New York.
Gabel, D. 1974. Active site titration of immobilized chymotrypsin with a fluorogenic reagent. FEBS Letters 49: 280–281.
Allcock, H.R. and Lampe, F.W. 1990. Contemporary Polymer Chemistry. PrenticeHall, Inc., Englewood Cliffs, NJ.
Wangikar, P.P., Michels, P.C., Clark, D.S. and Dordick, J.S. 1997. Structure and function of subtilisin BPŃ solubilized in organic solvents. J. Am. Chem. Soc. 119: 70–76.
Stepanov, V.M. 1996. Proteinases as catalysts in peptide synthesis. Pure and Applied Chemistry 68: 1335–1339.
Rich, J.O., Bedell, B.A. and Dordick, J.S. 1995. Controlling enzyme-catalyzed regioselectivity in sugar ester synthesis. Biotechnol. Bioeng. 45: 426–434.
Moris, F. and Gotor, V. 1993. A useful and versatile procedure for the acylation of nucleosides through an enzymatic reaction. J. Org. Chem. 58: 653–660.
Rich, J.O. and Dordiok, J.S. 1996. Controlling regioselectivity in enzyme-catalyzed acylation of polyhydroxyl compounds. Ann. N.Y. Acad. Sci. 799: 226–230.
Fields, R. 1971. The measurement of amino groups in proteins and peptides. Biochem. J. 124: 581–590.
Orthgeiss, E. and Dobias, B. 1990. Colorimetric determination of anionic surfactants. Biotechnol. Bioeng. 40: 91–102.
Wangikar, P.P., Carmichael, D., Clark, D.S. and Dordick, J.S. 1995. Active site titration of serine protaases in organic solvents. Biotechnol. Bioeng. 50: 329–335.
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Wang, P., Sergeeva, M., Lim, L. et al. Biocatalytic plastics as active and stable materials for biotransformations. Nat Biotechnol 15, 789–793 (1997). https://doi.org/10.1038/nbt0897-789
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DOI: https://doi.org/10.1038/nbt0897-789
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