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Use of a specific Monoclonal Antibody for the Preparation of a Highly Active Immobilized Carboxypeptidase A

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Abstract

A mouse monoclonal antibody (Ab 100) to carboxypeptidase A (CPA) which binds to the enzyme with a high affinity constant (∼109 M−1) without affecting its catalytic activity was prepared, purified and characterized. Covalent binding of the monoclonal antibody to Eupergit C, or noncovalent binding to Sepharose-Protein A, yielded carriers reacting specifically with CPA to give immobilized enzyme preparations displaying full catalytic activity and improved stability. The method represents a general technique for the preparation of highly active immobilized enzymes.

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Authors and Affiliations

  1. Center for Biotechnology, Tel-Aviv University, Tel-Aviv, 69978, Israel

    Beka Solomon, Rela Koppel & Ephraim Katchalski-Katzir

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  1. Beka Solomon
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  2. Rela Koppel
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  3. Ephraim Katchalski-Katzir
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Solomon, B., Koppel, R. & Katchalski-Katzir, E. Use of a specific Monoclonal Antibody for the Preparation of a Highly Active Immobilized Carboxypeptidase A. Nat Biotechnol 2, 709–712 (1984). https://doi.org/10.1038/nbt0884-709

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  • DOI: https://doi.org/10.1038/nbt0884-709

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