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Selective production of transgenic mice using green fluorescent protein as a marker

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Abstract

The low efficiency of transgenic animal production by microinjection has been a serious problem especially for the production of transgenic livestock. We developed a method to selectively produce transgenic mice using green fluorescent protein (GFP) as a marker. Using this method, we obtained eight fetuses and four live-born mice derived from 55 GFP-positive blastocysts. PCR analysis showed 11 out of 12 mice (fetuses and newborn mice) were transgenic. Southern blot analysis showed that 8 out of 12 were transgenic. GFP expression was also observed in bovine blastocysts, suggesting that this method should contribute to the efficient production of transgenic livestock.

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References

  1. Brinster, R.L., Chen, H.Y., Trumbauer, M.E., Yagle, M.K., and Palmiter, R.D. 1985. Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. Proc. Natl. Acad. Sci. USA 82: 4438–4442.

    Article  CAS  Google Scholar 

  2. Hammer, R.E., Pursel, V.G., Rexroad, C.E. Jr., Wall, R.J., Bolt, D.J., Ebert, K.M. et al. 1985. Production of transgenic rabbits, sheep and pigs by microinjection. Nature 315: 80–683.

    Google Scholar 

  3. Cubitt, A.B., Heim, R., Adams, S.R., Boyd, A.E., Gross, L.A., and Tsien, R.Y. 1995. Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci. 20: 448–455.

    Article  CAS  Google Scholar 

  4. Thompson, E.M., Adenot, P., Tsuji, F.I., and Renard, J.-P. 1995. Real time imaging of transcriptional activity in live mouse preimplantation embryos using a secreted luciferase. Proc. Natl. Acad. Sci. USA 92: 1317–1321.

    Article  CAS  Google Scholar 

  5. Ikawa, M., Kominami, K., Yoshimura Y., Tanaka, K., Nishimune, Y., and Okabe, M. 1995. A rapid and non-invasive selection of transgenic embryos before implantation using green fluorescent protein (GFP). FEBS Lett. 375: 125–128.

    Article  CAS  Google Scholar 

  6. Hanaoka, K., Hayasaka, M., Uetsuki, T., Fujisawa-Sehara, A., and Nabeshima, Y. 1991. A stable cellular marker for the analysis of mouse chimeras: the bacterial chloramphenicol acetyltransferase gene driven by the human elongation factor 1 a promoter. Differentiation 48: 183–189.

    Article  CAS  Google Scholar 

  7. Oshima, M., Oshima, H., Kobayashi, M., Tsutsumi, M., and Taketo, M.M. 1995. Evidence against dominant negative mechanisms of intestinal polyp formation by Ape gene mutations. Cancer Res. 55: 2719–2722.

    CAS  PubMed  Google Scholar 

  8. Niwa, H., Yamamura, K., and Miyazaki, J. 1991. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108: 193–200.

    Article  CAS  Google Scholar 

  9. Ikawa, M., Kominami, K., Yoshimura, Y., Tanaka, K., Nishimune, Y., and Okabe, M. 1995. Green fluorescent protein as a marker in transgenic mice. Develop. Growth Differ. 37: 455–459.

    Article  CAS  Google Scholar 

  10. King, D. and Wall, R.J. 1988. Identification of specific gene sequences in preimplantation embryos by genomic amplification: detection of a transgene. Mol. Reprod. Develop. 1: 57–62.

    Article  CAS  Google Scholar 

  11. Ninomiya T., Hoshi, M., Mizuno, A., Nagao, M., and Yuki, A 1989. Selection of mouse preimplantation embryos carrying exogenous DNA by polymerase chain reaction. Mol. Reprod. Develop. 1: 242–248.

    Article  CAS  Google Scholar 

  12. Heim, R., Cubitt, A.B., and Tsien, R.Y. 1995. Improved green fluorescence. Nature 373: 663–664.

    Article  CAS  Google Scholar 

  13. Kim, D.W., Uetsuki T., Kaziro, Y., Yamaguchi, N., and Sugano, S. 1990. Use of the human elongation factor 1 a promoter as a versatile and efficient expression system. Gene 91: 217–223.

    Article  CAS  Google Scholar 

  14. Uetsuki, T., Naito, A., Nagata, S., and Kaziro, Y. 1989. Isolation and characterization of the human chromosomal gene for polypeptide chain elongation factor 1α. J. Biol. Chem. 264: 5791–5798

    CAS  PubMed  Google Scholar 

  15. Hogan, B., Beddington, R., Costaniti, F., and Lacy, E. 1994. pp. 232–248 in Manipulating the mouse embryo: a laboratory manual. 2nd ed. Hogan, B., Beddington, R., Costaniti, F, and Lacy, E. (eds). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

    Google Scholar 

  16. Brackett, B.G. and Oliphant, G. 1975. Capacitation of rabbit spermatozoa in vitro. Biol. Reprod. 12: 260–274.

    Article  CAS  Google Scholar 

  17. Takahashi, Y. and First, N.L. 1992. In vitro development of bovine one-cell embryos: influence of glucose, lactate, pyruvate, amino acids and vitamins. Theriogenology 37: 963–978.

    Article  CAS  Google Scholar 

  18. Church, G.M. and Gilbert, W. 1984. Genomic sequencing. Proc. Natl. Acad. Sci. USA 81: 1991–1995.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Molecular, Cell Pharmacology, National Children?s Medical Research Center, Tokyo, 154, Japan

    Tatsuyuki Takada, Keiko lida, Takeo Awaji & Gozoh Tsujimoto

  2. YS New Technology Institute Inc., Tochigi, 329-05, Japan

    Kazumi Itoh & Riichi Takahashi

  3. Department of Virology, Institute of Medical Science, University of Tokyo, Tokyo, 108, Japan

    Akiko Shibui, Kenichi Yoshida & Sumio Sugano

Authors
  1. Tatsuyuki Takada
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  2. Keiko lida
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  3. Takeo Awaji
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  4. Kazumi Itoh
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  5. Riichi Takahashi
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  6. Akiko Shibui
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  7. Kenichi Yoshida
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  8. Sumio Sugano
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  9. Gozoh Tsujimoto
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Correspondence to Tatsuyuki Takada.

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Takada, T., lida, K., Awaji, T. et al. Selective production of transgenic mice using green fluorescent protein as a marker. Nat Biotechnol 15, 458–461 (1997). https://doi.org/10.1038/nbt0597-458

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  • DOI: https://doi.org/10.1038/nbt0597-458

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