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Synthesis and Assembly in Yeast of Hepatitis B Surface Antigen Particles Containing the Polyalbumin Receptor

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Abstract

Yeast cells are genetically engineered to synthesize and assemble hepatitis B surface antigen particles containing 55 additional amino acids encoded by the hepatitis B virus preS gene. These particles are composed of a 28,000 dalton protein and efficiently bind polymerized human albumin. Antibodies to the polyalbumin receptor epitope of this particle may interfere with the postulated polyalbumin-mediated binding of HBV to hepatocytes and may therefore be particularly efficient in preventing the disease.

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Authors and Affiliations

  1. Chiron Research Laboratories, Chiron Corporation, 4560 Horton Street, Emeryville, CA, 94608

    Pablo Valenzuela, Doris Coit & Ching H. Kuo

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  1. Pablo Valenzuela
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  2. Doris Coit
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  3. Ching H. Kuo
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Valenzuela, P., Coit, D. & Kuo, C. Synthesis and Assembly in Yeast of Hepatitis B Surface Antigen Particles Containing the Polyalbumin Receptor. Nat Biotechnol 3, 317–320 (1985). https://doi.org/10.1038/nbt0485-317

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  • DOI: https://doi.org/10.1038/nbt0485-317

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