A transcriptome can be readily assembled from RNA-Seq data in the absence of a reference genome.
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Iyer, M., Chinnaiyan, A. RNA-Seq unleashed. Nat Biotechnol 29, 599–600 (2011). https://doi.org/10.1038/nbt.1915
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DOI: https://doi.org/10.1038/nbt.1915
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