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Discrimination of Heterozygous from Wild-Type Mice Bearing a Mutation in the Macrophage–Colony-Stimulating Factor Gene Using Capillary Electrophoresis

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Abstract

Discriminating between phenotypically indistinguishable heterozygous and wild-type mice presents a difficult situation for researchers attempting to find suitable breeding pairs. The authors describe a fast, reliable, nonradioactive method that combines PCR with capillary electrophoresis to determine genotypical differences among heterozygous (+/op), homozygous (op/op), and wild-type (+/+) mice.

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Figure 1: M-CSF sequence of a 134-bp product generated from mice genomic DNA.
Figure 2: Electropherograms from heterozygous, homozygous, and wild-type mice.

References

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Acknowledgements

The authors thank Luisita Egger (Institute of Legal Medicine, Zentralkrankenhaus St. Jürgen Strasse, Bremen, Germany) and Stephanie Fischer (Working Group for Experimental and Clinical Arteriogenesis at the Department for Internal Medicine III, Albert-Ludwigs University of Freiburg, Germany) for their superb technical assistance. A grant from the Volkswagen Foundation supported this study.

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Authors and Affiliations

  1. Techel is at the Institute of Legal Medicine, Central Bremen Clinic, 28205 Bremen, Germany.,

    Dieter Techel PhD

  2. and Jost are in the Working Group for Experimental and Clinical Arteriogenesis at the Department for Internal Medicine III, Bergmann, Buschmann, Albert-Ludwigs University of Freiburg, 79106 Freiburg, Germany.,

    Caroline Bergmann DVM, Ivo Buschmann MD & Marco M. Jost PhD

  3. Jost is also affiliated with BioVisioN AG, 30625 Hannover, Germany.,

    Marco M. Jost PhD

Authors
  1. Dieter Techel PhD
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  2. Caroline Bergmann DVM
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  3. Ivo Buschmann MD
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  4. Marco M. Jost PhD
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Corresponding author

Correspondence to Dieter Techel PhD.

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Techel, D., Bergmann, C., Buschmann, I. et al. Discrimination of Heterozygous from Wild-Type Mice Bearing a Mutation in the Macrophage–Colony-Stimulating Factor Gene Using Capillary Electrophoresis. Lab Anim 33, 33–35 (2004). https://doi.org/10.1038/laban0904-33

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  • DOI: https://doi.org/10.1038/laban0904-33

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