Skip to main content
SpringerLink
Log in

Dual Tail Catheters for Infusion and Sampling in Rats as an Efficient Platform for Metabolic Experiments

  • Technique
  • Published:
Lab Animal

Abstract

The authors present a technique for establishing a virtually stress-free dual-catheter system in rats, allowing researchers to conduct extended, complex experimental procedures involving the simultaneous infusion of multiple compounds and frequent collection of blood samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: Progression of a hyperinsulin-emic-euglycemic clamp study.

References

  1. Nachtman, R.G., Driscoll, T.B., Gibson, L.A. & Johnson, P.C. Jr. Commercial over-the-needle catheters for intravenous injections and blood sampling in rats. Lab. Anim. Sci. 38, 629–630 (1988).

    CAS  PubMed  Google Scholar 

  2. Kraegen, E.W., James, D.E., Bennett, S.P. & Chisholm, D.J. In vivo insulin sensitivity in the rat determined by euglycemic clamp. Am. J. Physiol. 245, E1–7 (1983).

    Article  CAS  Google Scholar 

  3. el-Shahawy, M.A. & Khilnani, H. Carotid-jugular arteriovenous fistula: a complication of temporary hemodialysis catheter. Am. J. Nephrol. 15, 332–336 (1995).

    Article  CAS  Google Scholar 

  4. Guo, Z. & Jensen, M.D. Blood glycerol is an important precursor for intramuscular triacylglycerol synthesis. J. Biol. Chem. 274, 23702–23706 (1999).

    Article  CAS  Google Scholar 

  5. Dworkin, B.R., Filewich, R.J., Da Costa, J., Eissenberg, E. & Miller, N.E. A chronic arterial catheter and low compliance system for recording blood pressure and heart rate from the rat. Am. J. Physiol. 239, H137–141 (1980).

    CAS  PubMed  Google Scholar 

  6. Golden, S., Chenoweth, M., Dunn, A., Okajima, F. & Katz, J. Metabolism of tritium- and 14C-labeled alanine in rats. Am. J. Physiol. 241, E121–128 (1981).

    Article  CAS  Google Scholar 

  7. Moslen, M.T., Kanz, M.F., Bhatia, J. & Catarau, E.M. Two cannula method for parenteral infusion and serial blood sampling in the freely moving rat. JPEN J. Parenter. Enterol. Nutr. 12, 633–637 (1988).

    Article  CAS  Google Scholar 

  8. Furuhama, K. & Onodera, T. A simple technique for repeated blood collection from the tail vein of the rat. J. Toxicol. Sci. 8, 161–163 (1983).

    Article  CAS  Google Scholar 

  9. Frank, P., Schoenhard, G.L. & Burton, E. A method for rapid and frequent blood collection from the rat tail vein. J. Pharmacol. Methods 26, 233–238 (1991).

    Article  CAS  Google Scholar 

  10. Fejes-Toth, G., Naray-Fejes-Toth, A., Roage, D. & Frolich, J.C. Chronic arterial and venous catheterization of conscious, unrestrained rats. Hypertension 6, 926–930 (1984).

    Article  CAS  Google Scholar 

  11. Hagmuller, K., Liebmann, P., Porta, S. & Rinner, I. A tail-artery cannulation method for the study of blood parameters in freely moving rats. J. Pharmacol. Toxicol. Methods 28, 79–83 (1992).

    Article  CAS  Google Scholar 

  12. Fluttert, M., Dalm, S. & Oitzl, M.S. A refined method for sequential blood sampling by tail incision in rats. Lab. Anim. (NY) 34, 372–378 (2000).

    Article  CAS  Google Scholar 

  13. Kim, J.K., Wi, J.K. & Youn, J.H. Plasma free fatty acids decrease insulin-stimulated skeletal muscle glucose uptake by suppressing glycolysis in conscious rats. Diabetes 45, 446–453 (1996).

    Article  CAS  Google Scholar 

  14. Sacca, L., Toffolo, G. & Cobelli, C. V-A and A-V modes in whole body and regional kinetics: domain of validity from a physiological model. Am. J. Physiol. 263, E597–606 (1992).

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The work was supported by the National Institute of Diabetes and Digestive and Kidney Diseases Grant DK 60013 and by a Pilot and Feasibility Program Project award from the Minnesota Obesity Center.

Author information

Authors and Affiliations

  1. Endocrine Research Unit, Mayo Foundation, Rochester, MN 55905

    ZengKui Guo PhD & Lianzhen Zhou Ph Lic

Authors
  1. ZengKui Guo PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lianzhen Zhou Ph Lic
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to ZengKui Guo PhD.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Guo, Z., Zhou, L. Dual Tail Catheters for Infusion and Sampling in Rats as an Efficient Platform for Metabolic Experiments. Lab Anim 32, 45–48 (2003). https://doi.org/10.1038/laban0203-45

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/laban0203-45

  • Springer Nature America, Inc.

This article is cited by

Search

Navigation