Abstract
The large disparity between arsenic concentrations in drinking water and urine remains unexplained. This study aims to evaluate predictors of urinary arsenic in a population exposed to low concentrations (≤50 μg/l) of arsenic in drinking water. Urine and drinking water samples were collected from a subsample (n=343) of a population enrolled in a bladder cancer case–control study in southeastern Michigan. Total arsenic in water and arsenic species in urine were determined using ICP-MS: arsenobetaine (AsB), arsenite (As[III]), arsenate (As[V]), methylarsenic acid (MMA[V]), and dimethylarsenic acid (DMA[V]). The sum of As[III], As[V], MMA[V], and DMA[V] was denoted as SumAs. Dietary information was obtained through a self-reported food intake questionnaire. Log10-transformed drinking water arsenic concentration at home was a significant (P<0.0001) predictor of SumAs (R2=0.18). Associations improved (R2=0.29, P<0.0001) when individuals with less than 1 μg/l of arsenic in drinking water were removed and further improved when analyses were applied to individuals who consumed amounts of home drinking water above the median volume (R2=0.40, P<0.0001). A separate analysis indicated that AsB and DMA[V] were significantly correlated with fish and shellfish consumption, which may suggest that seafood intake influences DMA[V] excretion. The Spearman correlation between arsenic concentration in toenails and SumAs was 0.36 and between arsenic concentration in toenails and arsenic concentration in water was 0.42. Results show that arsenic exposure from drinking water consumption is an important determinant of urinary arsenic concentrations, even in a population exposed to relatively low levels of arsenic in drinking water, and suggest that seafood intake may influence urinary DMA[V] concentrations.
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Acknowledgements
We appreciate the involvement of all study participants for taking part in this research. We also thank the collaboration of Aaron M. Linder, Lingling Zhang, Luis O. Rivera-González, Stacey Fedewa, Gillian AvRuskin, Angela Hungerink, Roni Kobrosly, Nicholas Mank, and Caitlyn Meservey for valuable assistance with different aspects of this project. This research was funded by the National Cancer Institute, Grant RO-1 CA96002-10. The first author received support from the Comprehensive Minority Biomedical Branch of the National Cancer Institute. The perspectives are those of the authors and do not necessarily represent the official position of the funding agency.
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Rivera-Núñez, Z., Meliker, J., Meeker, J. et al. Urinary arsenic species, toenail arsenic, and arsenic intake estimates in a Michigan population with low levels of arsenic in drinking water. J Expo Sci Environ Epidemiol 22, 182–190 (2012). https://doi.org/10.1038/jes.2011.27
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DOI: https://doi.org/10.1038/jes.2011.27
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