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Lipids and cardiovascular/metabolic health

Bioavailability of long-chain n-3 fatty acids from enriched meals and from microencapsulated powder

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Abstract

Background:

Despite the potential benefits of long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs), intake is often low because of low consumption of oily seafood. Microencapsulated fish oil powder can improve tolerance and acceptance of LC n-3 PUFAs. Bioavailability is important to achieve efficacy. We investigated the bioavailability of LC n-3 PUFAs from microencapsulated powder in comparison with meals enriched with liquid fish oil.

Methods:

Participants (N=99, age⩾50 years) of this 4-week double-blinded dietary intervention were randomized into three groups. Group 1 (n=38) received 1.5 g/d eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) as ready-to-eat meals enriched with liquid fish oil; group 2 (n=30) received the same amount of these LC n-3 PUFAs as microencapsulated fish oil powder and regular meals; and group 3 (n=31) was the control group, which received placebo powder and regular meals. Blood samples were taken from fingertips at baseline and at the end point.

Results:

Seventy-seven subjects (77.8%) completed the study. The amount of EPA in blood doubled in both groups that received LC n-3 PUFAs (P<0.05), but it did not change in the control group. The changes in DHA were less but still significant in both intervention groups. According to multivariate analysis, both intervention groups had higher end-point LC n-3 PUFA concentrations compared with placebo, but differences between intervention groups were not significant.

Conclusion:

Bioavailability of LC n-3 PUFAs in encapsulated powder is very similar to the bioavailability of LC n-3 PUFAs in ready-to-eat meals enriched with liquid fish oil. Thus, encapsulated powder can be considered useful to increase LC n-3 PUFA concentrations in blood.

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References

  1. Delgado-Lista J, Perez-Martinez P, Lopez-Miranda J, Perez-Jimenez F . Long chain omega-3 fatty acids and cardiovascular disease: a systematic review. Br J Nutr 2012; 107: S201–S213.

    Article  CAS  Google Scholar 

  2. Calder PC . Polyunsaturated fatty acids and inflammation. Prostaglandins Leukot Essent Fatty Acids 2006; 75: 197–202.

    Article  CAS  Google Scholar 

  3. Calder PC . Omega-3 fatty acids and inflammatory processes. Nutrients 2010; 2: 355–374.

    Article  CAS  Google Scholar 

  4. Han YY, Lai SL, Ko WJ, Chou CH, Lai HS . Effects of fish oil on inflammatory modulation in surgical intensive care unit patients. Nutr Clin Pract 2012; 27: 91–98.

    Article  Google Scholar 

  5. Nordic Councils of Ministers Nordic Nutrition Recommendations 2012: Integrating nutrition and physical activity. 5th ed Norden: Copenhagen, 2012.

  6. Nordic Councils of Ministers Nordic Nutrition Recommendations 2004: Integrating nutrition and physical activity. 4th ed Norden: Copenhagen, 2004.

  7. Thorgeirsdottir H, Valgersdottir H, Gunnarsdottir I, Gisladottir I, Gunnarsdottir BE, Thorsdottir I et al. The Diet of Icelanders. Dietary Survey of The Icelandic Directorate of Health 2010-2011. Main findings 2011.

  8. Steingrimsdottir L, Thorgeirsdottir H, Olafsdottir AS . The Diet of Icelanders. Dietary Survey of The Icelandic Nutrition Council 2002. Main findings 2002.

  9. Lane K, Derbyshire E, Li W, Brennan C . Bioavailability and potential uses of vegetarian sources of omega-3 fatty acids: a review of the literature. Crit Rev Food Sci Nutr. 2014; 54: 572–579.

    Article  CAS  Google Scholar 

  10. Metcalf RG, James MJ, Mantzioris E, Cleland LG . A practical approach to increasing intakes of n-3 polyunsaturated fatty acids: use of novel foods enriched with n-3 fats. Eur J Clin Nutr 2003; 57: 1605–1612.

    Article  CAS  Google Scholar 

  11. McCowen KC, Ling PR, Decker E, Djordjevic D, Roberts RF, Coupland JN et al. A simple method of supplementation of omega-3 polyunsaturated fatty acids: use of fortified yogurt in healthy volunteers. Nutr Clin Pract 2010; 25: 641–645.

    Article  Google Scholar 

  12. Schuchardt JP, Hahn A . Bioavailability of long-chain omega-3 fatty acids. Prostaglandins Leukot Essent Fatty Acids 2013; 89: 1–8.

    Article  CAS  Google Scholar 

  13. Kastelein JJ, Maki KC, Susekov A, Ezhov M, Nordestgaard BG, Machielse BN, Kling D, Davidson MH . Omega-3 free fatty acids for the treatment of severe hypertriglyceridemia: the EpanoVa fOr Lowering Very high triglyceridEs (EVOLVE) trial. J Clin Lipidol 2014; 8: 94–106.

    Article  Google Scholar 

  14. Wallace JM, McCabe AJ, Robson PJ, Keogh MK, Murray CA, Kelly PM et al. Bioavailability of n-3 polyunsaturated fatty acids (PUFA) in foods enriched with microencapsulated fish oil. Ann Nutr Metab 2000; 44: 157–162.

    Article  CAS  Google Scholar 

  15. Higgins S, Carroll YL, O’Brien NM, Morrissey PA . Use of microencapsulated fish oil as a means of increasing n-3 polyunsaturated fatty acid intake. J Hum Nutr Diet 1999; 12: 265–271.

    Article  Google Scholar 

  16. Saga LC, Liland KH, Leistad RB, Reimers A, Rukke EO . Relating fatty acid composition in human fingertip blood to age, gender, nationality and n-3 supplementation in the Scandinavian population. Int J Food Sci Nutr 2012; 63: 790–795.

    Article  CAS  Google Scholar 

  17. Marangoni F, Colombo C, Galli C . A method for the direct evaluation of the fatty acid status in a drop of blood from a fingertip in humans: applicability to nutritional and epidemiological studies. Anal Biochem 2004; 326: 267–272.

    Article  CAS  Google Scholar 

  18. Dyerberg J, Madsen P, Møller JM, Aardestrup I, Schmidt EB . Bioavailability of marine n-3 fatty acid formulations. Prostaglandins Leukot Essent Fatty Acids 2010; 83: 137–141.

    Article  CAS  Google Scholar 

  19. Wakil A, Mackenzie G, Diego-Taboada A, Bell JG, Atkin SL . Enhanced bioavailability of eicosapentaenoic acid from fish oil after encapsulation within plant spore exines as microcapsules. Lipids 2010; 45: 645–649.

    Article  CAS  Google Scholar 

  20. Metherel AH, Armstrong JM, Patterson AC, Stark KD . Assessment of blood measures of n-3 polyunsaturated fatty acids with acute fish oil supplementation and washout in men and women. Prostaglandins Leukot Essent Fatty Acids 2009; 81: 23–29.

    Article  CAS  Google Scholar 

  21. Harris WS, Von Schacky C . The Omega-3 Index: a new risk factor for death from coronary heart disease? Prev Med 2004; 39: 212–220.

    Article  CAS  Google Scholar 

  22. Harris WS . The omega-3 index as a risk factor for coronary heart disease. Am J Clin Nutr 2008; 87: 1997–2002.

    Article  Google Scholar 

  23. Siscovick DS, Raghunathan TE, King I, Weinmann S, Wicklund KG, Albright J et al. Dietary intake and cell membrane levels of long-chain n-3 polyunsaturated fatty acids and the risk of primary cardiac arrest. JAMA 1995; 274: 1363–1367.

    Article  CAS  Google Scholar 

  24. Harris WS, Poston WC, Haddock CK . Tissue n-3 and n-6 fatty acids and risk for coronary heart disease events. Atherosclerosis 2007; 193: 1–10.

    Article  CAS  Google Scholar 

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Acknowledgements

This project was funded by Nordic Innovation no 11057: Enriched Convenience Seafood Products. The test meals were provided by Grimur Kokkur ehf, Vestmanneyjar, Iceland; the microencapsulated powder was provided by BioActive Foods AS, Trondheim, Norway. None of these three entities participated in data collection, data interpretation or publication.

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Correspondence to A Ramel.

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Hinriksdottir, H., Jonsdottir, V., Sveinsdottir, K. et al. Bioavailability of long-chain n-3 fatty acids from enriched meals and from microencapsulated powder. Eur J Clin Nutr 69, 344–348 (2015). https://doi.org/10.1038/ejcn.2014.250

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  • DOI: https://doi.org/10.1038/ejcn.2014.250

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