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Clonogenic assays in the B16 melanoma: response to cyclophosphamide

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Abstract

The survival of clonogenic cells in the B16 melanoma has been studied simultaneously by 3 methods: an in vitro assay in soft agar, a lung-colony assay, and the end-point dilution technique. Details of the first 2 methods have previously been reported, but those of the third are described here. The 3 methods have agreed well in investigations of the response of the B16 melanoma to cyclophosphamide.

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Authors
  1. G G Steel
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  2. K Adams
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  3. T C Stephens
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Steel, G., Adams, K. & Stephens, T. Clonogenic assays in the B16 melanoma: response to cyclophosphamide. Br J Cancer 36, 618–624 (1977). https://doi.org/10.1038/bjc.1977.239

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  • DOI: https://doi.org/10.1038/bjc.1977.239

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