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Imaging mass spectrometry: A new technology for the analysis of protein expression in mammalian tissues

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Figure 1: Methodology developed for the spatial analysis of tissue by MALDI mass spectrometry.
Figure 2: Mass spectrometric images of a mouse brain section.
Figure 3: Selected protein images from a glioblastoma section.
Figure 4: MALDI mass spectra taken at different locations within a glioblastoma slice (Fig. 3).
Figure 5: UV chromatogram of a LC separation on a glioblastoma xenograft extract.
Figure 6: The mass spectrometric analysis by electrospray MS/MS of the N-terminal tryptic fragment of Tβ.4.

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Acknowledgements

We thank S. Schroeter, E. Sierra-Rivera, B. DaGue and Darell Bigner for help with this study. This work was supported by NIH grants GM 58008 (to R.M.C.), CA 58506 (to D.H.) and C.A. 70937 (to D.H.).

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  1. Mass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, Tennessee, USA

    Markus Stoeckli, Pierre Chaurand, Dennis E. Hallahan & Richard M. Caprioli

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  1. Markus Stoeckli
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  2. Pierre Chaurand
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  4. Richard M. Caprioli
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Correspondence to Richard M. Caprioli.

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Stoeckli, M., Chaurand, P., Hallahan, D. et al. Imaging mass spectrometry: A new technology for the analysis of protein expression in mammalian tissues. Nat Med 7, 493–496 (2001). https://doi.org/10.1038/86573

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