Skip to main content

Advertisement

Log in

Viral receptors and vector purification: New approaches for generating clinical-grade reagents

  • On the Market
  • Published:

From Nature Medicine

View current issue Submit your manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Figure 1: Heparan sulfate proteoglycan is a receptor for AAV-2 virions.
Figure 2: New strategies for purification of AAV vector.

References

  1. Friedmann, T. in The Development of Human Gene Therapy (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1999).

    Google Scholar 

  2. Williamsburg BioProcessing Foundation Meeting, Viral Vectors and Vaccines, (The Williamsburg Bioprocessing Foundation, Norfolk, Virginia,1998).

  3. Summerford, C. & Samulski, R.J. Membrane-associated heparan sulfate proteoglycan is a receptor for adeno-associated virus type 2 virions. J. Virol. 72, 1438–1445 (1998).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Wistuba, A., Kern, A., Weger, S., Grimm, D. & Kleinschmidt, J.A. Subcellular compartmentalization of adeno-associated virus type 2 assembly. J. Virol. 71, 1341–1352 (1997).

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Grimm, D., Kern, A., Rittner, K. & Kleinschmidt, J.A. Novel tools for production and purification of recombinant adeno-associated virus vectors. Hum. Gene Ther. 9, 2745–2760 (1998).

    Article  CAS  Google Scholar 

  6. Zolotukhin, S. et al. Recombinant adeno-associated virus purification using novel methods improves infectious titer and yield. Gene Ther. (in the press).

  7. Clark, K.R., Liu, X., McGrath, J.P. & Johnson, P.R. Highly purified recombinant adeno-associated virus vectors are biologically active and free of detectable helper and wild type viruses. Hum. Gene Ther. (in the press).

  8. Xiao, X., Li, J. & Samulski, R.J. Production of high titer recombinant adeno-associated virus vectors in the absence of helper adenovirus. J. Virol. 72, 2224–2232 (1998).

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Clark, K.R., Voulgaropoulou, F., Fraley D.M., & Johnson, P.R. Cell lines for the production of infectious recombinant adeno-associated viruses. Hum. Gene Ther. 6, 1329–1341 (1995).

    Article  CAS  Google Scholar 

  10. Summerford, C., Bartlett, J.S. & Samulski, R.J. αVβ5 integrin: A co-receptor for adeno-associated virus type 2 infection. Nature Med. 5, 78–81 (1999).

    Article  CAS  Google Scholar 

  11. Qing, K. et al. Human fibroblast growth factor receptor 1 is a co-receptor for infection by adeno-associated virus 2. Nature Med. 5, 71–77 (1999).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Richard Jude Samulski.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Summerford, C., Samulski, R. Viral receptors and vector purification: New approaches for generating clinical-grade reagents. Nat Med 5, 587–588 (1999). https://doi.org/10.1038/8470

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/8470

  • Springer Nature America, Inc.

This article is cited by

Navigation