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Protein production: feeding the crystallographers and NMR spectroscopists

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Abstract

Protein purification efforts for structural genomics will focus on automation for the readily-expressed proteins, and process development for the more difficult ones, such as membrane proteins. Thousands of proteins are expected to be produced in the next few years. The purified proteins will be valuable reagents for the entire research community.

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Acknowledgements

The authors would like to acknowledge the research support of the Ontario government, the University of Toronto and the Ontario Cancer Institute. A.M.E. and C.H.A. are Scientists of the Canadian Institutes of Health Research (CIHR). J.F.G. is a Distinguished Scientist of the CIHR and a Foreign Investigator of the Howard Hughes Medical Institute. D.C. was supported by a Best Fellowship.

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Authors and Affiliations

  1. Ontario Cancer Institute, 610 University Avenue, Toronto, Ontario, Canada

    Aled M. Edwards, Cheryl H. Arrowsmith & Dinesh Christendat

  2. Banting and Best Department of Medical Research, University of Toronto, Toronto, M5W 1L6, Ontario, Canada

    Aled M. Edwards, James D. Friesen & Jack F. Greenblatt

  3. Integrative Proteomics, 161 Bay St., Toronto, Ontario, Canada

    Aled M. Edwards, Cheryl H. Arrowsmith, Akil Dharamsi, James D. Friesen, Jack F. Greenblatt & Masoud Vedadi

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  1. Aled M. Edwards
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  2. Cheryl H. Arrowsmith
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  3. Dinesh Christendat
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  4. Akil Dharamsi
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  5. James D. Friesen
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  6. Jack F. Greenblatt
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  7. Masoud Vedadi
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Correspondence to Aled M. Edwards.

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Edwards, A., Arrowsmith, C., Christendat, D. et al. Protein production: feeding the crystallographers and NMR spectroscopists . Nat Struct Mol Biol 7 (Suppl 11), 970–972 (2000). https://doi.org/10.1038/80751

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