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Selection of high-affinity phage antibodies from phage display libraries

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Abstract

Successful screening of phage display libraries will lead to more efficient, inexpensive antibody production.

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Figure 1: Procedure and experimental results for selection of displayed antibodies.
Figure 2: Monitoring insert size in phagemid populations.
Figure 3: Performance of anti-NH3 phages isolated by high-stringency elution.

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Acknowledgements

We are grateful to Greg Winter and collaborators for providing the Griffin.1 library and to Rosella Franconi and Eugenio Benvenuto for helpful discussions.

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Authors and Affiliations

  1. Department of developmental genetics, Institute for Molecular Biological Sciences, Vrije Universiteit, BioCentrum Amsterdam, de Boelelaan 1087, Amsterdam, 1081 HV, The Netherlands

    Robert de Bruin, Kees Spelt, Joseph Mol, Ronald Koes & Francesca Quattrocchio

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  1. Robert de Bruin
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  2. Kees Spelt
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  3. Joseph Mol
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  4. Ronald Koes
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  5. Francesca Quattrocchio
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Correspondence to Francesca Quattrocchio.

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Bruin, R., Spelt, K., Mol, J. et al. Selection of high-affinity phage antibodies from phage display libraries. Nat Biotechnol 17, 397–399 (1999). https://doi.org/10.1038/7959

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