Phosphoribosylanthranilate isomerase (PRAI) and indole-3-glycerol phosphate synthase (IGPS) are distantly related α/β-barrel enzymes that catalyze successive steps in tryptophan biosynthesis. A ‘new’ PRAI was evolved from an ‘old’ IGPS by random mutagenesis followed by DNA shuffling and in vivo selection.
References
Altamirano, M.M., Blackburn, J.M., Aguayo, C. & Fersht, A.R., Nature, 403, 617–622 (2000).
Wilmanns, M., Priestle, J.P., Niermann, T. & Jansonius, J.N., J. Mol. Biol. 223, 477–507 (1992).
Hyde, C.C., Ahmed, S.A., Padlan, E.A., Miles, E. W. & Davies, D.R., J. Biol. Chem. 263, 17857–17871 (1988).
Horowitz, N.H. Proc. Nat. Acad. Sci. USA 31, 153–157 (1945).
Reardon, D. & Farber, G.K., FASEB J. 9, 497–503 (1995).
Fani, R., Mori, E., Tamburini, E. & Lazcano, A., Orig. life Evol. Biosph. 28, 555–570 (1998).
Babbitt, P.C., et al. Biochemistry 35, 16489–16501 (1996).
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Gerlt, J. New wine from old barrels. Nat Struct Mol Biol 7, 171–173 (2000). https://doi.org/10.1038/73249
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DOI: https://doi.org/10.1038/73249
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